Immunohistochemistry analysis of Pygo2 expression in central nervous system tumors

  • Yi Liang
  • Chaoxi Wang
  • Apeng Chen
  • Lei Zhu
  • Jie Zhang
  • Pucha Jiang
  • Qiaoxin Yue
  • Gejing DeEmail author
Research Article


Pygo2 as a Wnt signaling pathway component has been detected in multiple cancer types. In this study, we identified Pygo2 expression features by immunohistochemistry in 73 central nervous system tumor specimens, in comparison with 14 normal brain tissues and surrounding non-tumorous tissues of tumor. Our study indicated that 59% of the patient tumor specimens exhibited positive Pygo2-staining and increases intensity with the grade of malignancy, especially for WHO grade III and IV gliomas, was observed high level expression, compared with normal brain tissues. Five out of nine WHO grade III anaplastic astrocytomas and seven out of nine WHO grade IV glioblastomas showed Pygo2-positive staining. The analysis of Pygo2 gene expression by quantitative real-time PCR of additional ten fresh patient samples yielded similar results. Further studies performed with stable cell lines in vitro demonstrated that Pygo2 render cells higher proliferation rate, migration and anchorage-independent colony-forming ability in soft agar. Taken together, our studies suggest an important role of Pygo2 in brain tumor progression.


Pygo2 Gliomas Immunohistochemistry Glioblastomas 



T cell factor


Lymphoid enhancer binding factor


Phosphate-buffered saline

PHD finger

Plant homeodomain finger



This research was supported by The National Natural Science Foundation of China (Grant No. 31501126). We thank Chao Ma, Heng Wei, and Feng Chen for supplying samples and medical assistance.

Supplementary material

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Copyright information

© The International CCN Society 2018

Authors and Affiliations

  1. 1.Laboratory Medicine CollegeGuangdong Medical UniversityGuangdongPeople’s Republic of China
  2. 2.Department of Neurological Surgery, School of MedicineUniversity of PittsburghPittsburghUSA
  3. 3.State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China
  4. 4.Department of NeurosurgeryZhongnan Hospital of Wuhan UniversityWuhanPeople’s Republic of China
  5. 5.Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijingPeople’s Republic of China

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