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Journal of Cell Communication and Signaling

, Volume 11, Issue 3, pp 245–253 | Cite as

The apoptotic inducible effects of salicylic acid on hepatoma cell line: relationship with nitric oxide signaling

  • Yahui Liu
  • Yong Wang
  • Yue Hu
  • Shuxiong Ge
  • Keshi Li
  • Shuangshuang Wang
  • Li Li
RESEARCH ARTICLE
  • 213 Downloads

Abstract

Clinical and experimental data suggest that salicylic acid (SA) is tumor preventive and NO has a multitude of effects on tumor biology. Therefore, firstly, the aim of our study is to explore the important role of SA in apoptotic induction of liver cancer cells. Secondly, we investigate whether SA mediates the anti-tumor effects by NO signaling pathway. The liver cancer cell line was treated with different concentrations of SA. Cell proliferation was tested using MTS assay and cell apoptosis was assessed by flow cytometry. NO content and NOS activities were measured by biochemical assay. The anti- or pro-apoptotic regulator gene expressions were analyzed by real-time PCR. Our data illustrated that high concentration of SA significantly inhibited liver cancer cell proliferation accompanied by apoptosis induction. In addition, SA led to the release of NO and the increase of NOS activities in above process. Importantly, SA up-regulated a series of apoptosis-related gene expression and reduced the mRNA level of HMGB1. Meanwhile, we also found that NOS inhibitor L-NAME and NO scavenger cPTIO attenuated the above SA-induced effects. Thus, we provided the evidence that SA exerted anti-tumor effects in liver cancer cell in part mediated by the NO pathway.

Keywords

Salicylic acid Apoptotic inducible effect Hepatoma cell line Nitric oxide 

Abbreviations

SA

Salicylic acid

NO

Nitric oxide

NOS

Nitric oxide synthase

L-NAME

NG-nitro-l-arginine methyl ester hydrochloride

cPTIO

2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt

Notes

Acknowledgements

This work was supported by Ningbo Medical Project Foundation (No. 2011B05) and the Major Science and Technology Planning Program of Ningbo (No. 2012C50013).

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Copyright information

© The International CCN Society 2017

Authors and Affiliations

  1. 1.Ningbo First HospitalNingbo CityPeople’s Republic of China
  2. 2.Ningbo UniversityNingboChina

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