FAT4 hypermethylation and grade dependent downregulation in gastric adenocarcinoma

  • Maryam Pilehchian Langroudi
  • Novin Nikbakhsh
  • Ali Akbar Samadani
  • Sadegh Fattahi
  • Hassan Taheri
  • Shahryar Shafaei
  • Galia Amirbozorgi
  • Reza Pilehchian Langroudi
  • Haleh Akhavan-NiakiEmail author


Gastric cancer is one of the major causes of death due to cancer in the world. It is a multi-factorial disease with epigenetic factors being also involved in its development. FAT4 is a tumor suppressor gene exerting an important role in cell adhesion. This study aimed at analyzing FAT4 expression and promoter methylation in gastric cancer. FAT4 expression was studied in 30 tumoral tissues and their non-tumoral counterparts using Taqman real time PCR method. Promoter methylation was assessed using bisulfite conversion method followed by sequencing. Tumor tissues showed reduced FAT4 expression (P = 0.04). FAT4 downregulation was associated with tumor grade, with higher repression at advanced grades. Significant increase of promoter methylation was observed in tumoral tissues. Reduced expression of FAT4 and increased methylation of its promoter may be one of the effective processes in turning a healthy stomach tissue into a tumor tissue.


Gastric cancer FAT4 Gene expression Promoter methylation 



This work was supported by the Research Deputy of Babol University of Medical Sciences. Authors would like to express their gratitude to the director and staffs of Razi Pathobiology laboratory, Babol, Iran, and North Research Center of Pasteur Institute, Amol, Iran for providing necessary instruments for accomplishing some experimental parts of this study. We appreciate the helpful comments from the staff members of the said university, research center and laboratory.

Compliance with ethical standards

Conflict of interest

Authors declare no conflict of interest.


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Copyright information

© The International CCN Society 2016

Authors and Affiliations

  • Maryam Pilehchian Langroudi
    • 1
    • 2
  • Novin Nikbakhsh
    • 3
  • Ali Akbar Samadani
    • 2
  • Sadegh Fattahi
    • 2
  • Hassan Taheri
    • 4
  • Shahryar Shafaei
    • 5
  • Galia Amirbozorgi
    • 6
  • Reza Pilehchian Langroudi
    • 7
  • Haleh Akhavan-Niaki
    • 1
    • 2
    Email author
  1. 1.Cellular and Molecular Biology Research CenterBabol University of Medical SciencesBabolIran
  2. 2.Department of Genetics, Faculty of MedicineBabol University of Medical SciencesBabolIran
  3. 3.Department of Surgery, Rouhani hospitalBabol University of Medical SciencesBabolIran
  4. 4.Department of Internal Medicine, Rouhani hospitalBabol University of Medical SciencesBabolIran
  5. 5.Department of Pathology, Rouhani hospitalBabol University of Medical SciencesBabolIran
  6. 6.North Research Center of Pasteur InstituteAmolIran
  7. 7.Department of Anaerobic Vaccine Research and ProductionRazi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO)KarajIran

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