Chemosensory Perception

, Volume 1, Issue 2, pp 153–162 | Cite as

Role of Odorant Binding Proteins: Comparing Hypothetical Mechanisms with Experimental Data

  • Andrew J. Taylor
  • David J. Cook
  • David J. Scott
Article

Abstract

Odorant binding proteins are expressed in the olfactory epithelium of many mammalian species and are thought to assist in the uptake and transfer of odorants from the gas phase to the odor receptors. Various mechanisms have been proposed to explain how mass transfer occurs. Most experimental work has focused on the binding of ligands to odorant binding proteins or to the olfactory receptors under steady-state conditions, whereas the situation in vivo is dynamic due to the tidal flow of air through the nose and the mass transfer in and out of the olfactory epithelium. Some preliminary dynamic data have been obtained from an in vitro system, and this has been used, along with other published data, to test some of the proposed mechanisms for odorant binding protein (OBP). An assessment of the hypothetical mechanisms has been made to examine how well the mechanisms fit with the published experimental data. A new hypothesis is proposed where OBP acts to prolong the odor signal and increases the signal output of the olfactory system.

Keywords

Mass Transfer Ligand Odorant Release Uptake Model System Olfaction OBP 

Notes

Acknowledgments

We thank Loic Briand and Jean-Claude Pernollet (INRA Jouy-en-Josas, France) for generously supplying OBP and helping in setting up OBP production in our laboratory as well as discussing the merits of the different OBP binding assays. An anonymous reviewer helpfully suggested the idea of ciliary action and its potential effect on mass transfer. The UK research council, BBSRC, provided funding for the practical part of the project.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Andrew J. Taylor
    • 1
  • David J. Cook
    • 1
  • David J. Scott
    • 1
  1. 1.School of BiosciencesUniversity of NottinghamLeicestershireUK

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