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Laser capture microdissection: techniques and applications in liver diseases

  • Beatriz Aguilar-Bravo
  • Pau Sancho-Bru
Review Article

Abstract

Routine transcriptomic and proteomic analysis are usually performed at a whole organ or tissue level. These approaches provide an average readout of all cell types present within the tissue but do not allow differentiating the profile of specific cell populations. Laser capture microdissection (LCM) constitutes an excellent tool to isolate cell populations or areas of interest within a tissue. By direct visualization, the selected area is excised by a laser and can be further processed for a variety of downstream analyses. This technology has been widely used in the study of liver diseases, from DNA and RNA sequencing to mass spectrometry. However, LCM also has important limitations. To ensure the best integrity of the molecule of interest, optimal tissue preservation, careful tissue sectioning, and optimization of the staining procedure are required. The present review provides a description of the LCM technology, including tips and technical recommendations to perform the procedure, as well as an overview of studies using LCM technology in the field of liver disease.

Keywords

Laser capture microdissection Liver diseases Tissue preservation Transcriptome Proteome Downstream analysis 

Notes

Acknowledgements

The work was funded by grants from Instituto de Salud Carlos III (PI17/00673), Miguel Servet (CP/00041) and PFIS (FI16/00203), co-financed by Fondo Europeo de Desarrollo Regional (FEDER), Unión Europea, ‘Una manera de hacer Europa’ and The European Foundation for Alcohol Research (ERAB) Grant EA1653.

Compliance with ethical standards

Funding

The work was funded by grants from the Instituto de Salud Carlos III (PI17/00673), Miguel Servet (CP/00041) and PFIS (FI16/00203), co-financed by the Fondo Europeo de Desarrollo Regional (FEDER), Unión Europea, ‘Una manera de hacer Europa’ and The European Foundation for Alcohol Research (ERAB) Grant EA1653.

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This article does not contain any study with human participants or animals performed by any of the authors.

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Copyright information

© Asian Pacific Association for the Study of the Liver 2019

Authors and Affiliations

  1. 1.Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS)BarcelonaSpain
  2. 2.Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)BarcelonaSpain

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