Human parenchymal and non-parenchymal liver cell isolation, culture and characterization
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Many reports describing parenchymal liver cell isolation have been published so far. However, recent evidence has clearly demonstrated that non-parenchymal liver cells play an important role in many pathophysiologies of the liver, such as drug-induced liver diseases, inflammation, and the development of liver fibrosis and cirrhosis. In this study, we present an overview of the current methods for isolating and characterizing parenchymal and non-parenchymal liver cells.
KeywordsHuman hepatocytes Liver cell isolation Parenchymal liver cells Non-parenchymal liver cells Liver cell culture
List of abbreviations
Dulbecco`s modified Eagle’s medium
Ethylene glycol tetraacetic acid
Fluorescence-activated cell sorting
Fetal calf serum
Glial fibriallary acidic protein
Hepatic stellate cells
Low density lipoprotein
Liver sinusoidal endothelial cells
Sinusoidal endothelial cells
This study was partially supported by BMBF-0315741 (Virtual Liver, Dr. Georg Damm/Prof. Andreas K. Nüssler), BMBF-0316058A (KIT-Chip, Prof. Andreas K. Nüssler), and BMBF-0315753 (Virtual Liver, Prof. Thomas S. Weiss).
Conflicts of Interest
Georg Damm, Elisa Pfeiffer, Britta Burkhardt, Jan Vermehren, Andreas K. Nüssler and Thomas S. Weiss declare that they have no conflicts of interest.
Compliance with Ethical Requirements
In the studies with human subjects carried out by the authors that are mentioned in this review, all procedures met the ethical standards of the responsible committee on human experimentation and with the Helsinki Declaration of 1975, as revised in 2008. Informed consent was obtained from all patients for inclusion in the study. Concerning the studies with animals carried out by the authors that are mentioned in this review, all institutional and national guidelines for the care and use of laboratory animals were followed.
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