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Hepatology International

, Volume 4, Issue 3, pp 601–607 | Cite as

Hepatitis B virus genotyping by enzyme-linked immunosorbent assay in Taiwan

  • Shu-Fen Liu
  • Meng-Hsuan Hsieh
  • Nai-Jen Hou
  • Ming-Yen Hsieh
  • Jee-Fu Huang
  • Chia-Yen Dai
  • Ming-Lung Yu
  • Wan-Long Chuang
Original Article

Abstract

Purpose

Restriction fragment length polymorphism (RFLP) and enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies (mAbs) were used in this study to detect genotypes of HBV, and the efficiency and precision of ELISA using the mAbs for HBV genotype detection were also estimated.

Methods

The ELISA with mAbs method was used for the detection of HBV genotype in a Taiwanese population. The HBV genotypes of 100 chronic hepatitis B patients were determined by ELISA and were then compared with those obtained using RFLP.

Results

Genotype B was found to be the most prevalent in this study (63% by RFLP; 62% by ELISA) followed by genotype C (31% by RFLP; 35% by ELISA). There was no significant difference between the results obtained by RFLP and ELISA (P = 0.75). The ELISA overall genotypeable rate, the correct genotyping rate from genotypeable specimens, and the concordance of the HBV genotyping assay was 96.00, 94.79, and 91.00%; for the ELISA HBV genotyping assay for genotype B specimens was 96.77, 100.00, and 96.77%; and for genotype C specimens was 97.14, 91.18, and 88.57%, respectively. The mean HBV DNA level was higher in the specimens that could be genotyped by both RFLP and ELISA samples (6.24 ± 1.77 vs. 2.34 ± 0.90, log IU/ml), and a significant difference in terms of HBV DNA level of more than 2 × 103 IU/ml was identified between the genotyped RFLP samples (P < 0.001).

Conclusions

ELISA is a practical and a useful method for HBV genotyping in a clinical setting in Taiwan, in particular for patients with lower levels of HBV DNA.

Keywords

Hepatitis B virus Genotype ELISA RFLP 

Notes

Acknowledgements

This study was supported by grants from the Taiwan Liver Research Foundation, Taiwan.

Conflict of interest

None.

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Copyright information

© Asian Pacific Association for the Study of the Liver 2010

Authors and Affiliations

  • Shu-Fen Liu
    • 1
  • Meng-Hsuan Hsieh
    • 2
  • Nai-Jen Hou
    • 3
  • Ming-Yen Hsieh
    • 4
  • Jee-Fu Huang
    • 3
    • 4
  • Chia-Yen Dai
    • 1
    • 4
  • Ming-Lung Yu
    • 1
    • 4
  • Wan-Long Chuang
    • 1
    • 4
  1. 1.Hepatobiliary Division, Department of Internal MedicineKaohsiung Medical University HospitalKaohsiungTaiwan
  2. 2.Department of Occupational MedicineKaohsiung Municipal HsiaoKang HospitalKaohsiungTaiwan
  3. 3.Department of Internal MedicineKaohsiung Municipal HsiaoKang HospitalKaohsiungTaiwan
  4. 4.Faculty of Internal Medicine, College of MedicineKaohsiung Medical UniversityKaohsiungTaiwan

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