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Theory in Biosciences

, Volume 132, Issue 1, pp 61–64 | Cite as

The rate of opening and closing of the DNA gate for topoisomerase II

  • Hui Ding
  • Hao Lin
  • Juan Feng
Short Communication

Abstract

Type II DNA topoisomerases can catalyze the transport of one DNA segment through a transient break in another DNA segment by a complex mechanism of ATP hydrolysis. According to the hydrolysis process of two ATPs, a multi-state model is proposed to investigate the work cycle of DNA topoisomerase II. The rate of the opening and closing of the DNA topoisomerase gate is evaluated by determining the release rate of inorganic phosphates. The calculated results show that, under the condition of the high concentration of ATP, the work cycle of DNA topoisomerase II is about 0.84 s which is in agreement with the experimental data.

Keywords

Type II DNA topoisomerases ATP release The rate of the opening and closing Master equation theory 

Notes

Acknowledgments

Supported by the National Natural Science Foundation of China (Grant No. 11047180 and 20973034), the Fundamental Research Funds for the Central Universities (ZYGX2009J081) and Scientific Research Startup Foundation of UESTC.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Key Laboratory for NeuroInformation of Ministry of Education, Center of Bioinformatics, School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengduChina

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