Theory in Biosciences

, Volume 131, Issue 4, pp 253–264 | Cite as

A kinetic approach to assess oxidative metabolism related features in the bivalve Mya arenaria

  • Paula Mariela González
  • Doris Abele
  • Susana PuntaruloEmail author
Original Paper


Electron paramagnetic resonance uses the resonant microwave radiation absorption of paramagnetic substances to detect highly reactive and, therefore, short-lived oxygen and nitrogen centered radicals. Previously, steady state concentrations of nitric oxide, ascorbyl radical (A·) and the labile iron pool (LIP) were determined in digestive gland of freshly collected animals from the North Sea bivalve Mya arenaria. The application of a simple kinetic analysis of these data based on elemental reactions allowed us to estimate the steady state concentrations of superoxide anion, the rate of A· disappearance and the content of unsaturated lipids. This analysis applied to a marine invertebrate opens the possibility of a mechanistic understanding of the complexity of free radical and LIP interactions in a metabolically slow, cold water organism under unstressed conditions. This data can be further used as a basis to assess the cellular response to stress in a simple system as the bivalve M. arenaria that can then be compared to cells of higher organisms.


Kinetic approach Ascorbyl radical Labile iron pool Superoxide anion Unsaturated fatty acids 



This study was supported by grants from the University of Buenos Aires, National Council for Science and Technology (CONICET) (PIP 1171) and DAAD-CONICET collaborative visit. S.P. is career investigator from CONICET and P.M.G. is a postdoctoral fellow from CONICET.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Paula Mariela González
    • 1
  • Doris Abele
    • 2
  • Susana Puntarulo
    • 1
    • 3
    Email author
  1. 1.Physical Chemistry-PRALIB, School of Pharmacy and BiochemistryUniversity of Buenos AiresBuenos AiresArgentina
  2. 2.Alfred Wegener Institute for Polar and Marine Research, Functional EcologyBremerhavenGermany
  3. 3.Fisicoquímica-PRALIB, Facultad de Farmacia y BioquímicaBuenos AiresArgentina

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