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Utilization of Advanced Backcross Population Derived from Synthetic Amphidiploid for Dissecting Resistance to Late Leaf Spot in Peanut (Arachis hypogaea L.)

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Late leaf spot (LLS) is the major foliar disease, which often occur with rust and cause severe yield losses worldwide in peanut (Arachis hypogaea L.). In order to map and introgress the resistance alleles for LLS into an elite cultivar ICGS 76, the advanced backcross approach was used for identification of quantitative trait locus (QTL). The synthetic amphidiploid ISATGR 278–18 (A. duranensis ICG 8138 × A. batizocoi ICG 13160) which is known to harbor resistance alleles for foliar diseases was used as donor parent to generate AB-QTL population comprising of 164 introgression lines (ILs). A linkage map with 114 microsatellite markers was developed spanning 746.15 cM distance with an average inter-marker distance of 6.55 cM. QTL analysis yielded 14 main-effect QTLs (mQTLs) (explaining up to 38.58% PVE) for LLS and 10 mQTLs (up to 10.99% PVE) for rust. In addition, four epistatic QTLs (eQTLs) (explaining up to 4.97% PVE) were observed for foliar diseases. Furthermore, seven mQTLs and 16 eQTLs were observed for agronomic traits. Several ILs showed high resistance to LLS with agronomic attributes from recurrent parent. This study reports the utilization of the untapped resistance source for LLS from A. batizocoi that was not extensively used earlier in peanut crop improvement.

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Fig. 1
Fig. 2



Quantitative trait loci


Introgression lines


Plant height


leaf length


leaf width


number of primary branches


number of secondary branches


shelling percent


total pod weight


test weight


rust scored after 80 days after sowing


rust scored 90 days after sowing


late leaf spot scored 80 days after sowing


late leaf spot scored 90 days after sowing


Phenotypic coefficient of variance,


Genotypic coefficient of variance

h2bs. %:

Broad sense heritability


Genetic advance over mean


Advanced backcross quantitative trait loci


phenotypic variance explained in percent


days after sowing


seasons are rainy seasons


is post-rainy season


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Kumari, V., Gowda, M.V.C. & Yeri, S.B. Utilization of Advanced Backcross Population Derived from Synthetic Amphidiploid for Dissecting Resistance to Late Leaf Spot in Peanut (Arachis hypogaea L.). Tropical Plant Biol. 13, 50–61 (2020).

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  • Advanced backcross
  • Genetic linkage map
  • Peanut
  • Synthetic amphidiploids
  • QTL