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Utilization of Advanced Backcross Population Derived from Synthetic Amphidiploid for Dissecting Resistance to Late Leaf Spot in Peanut (Arachis hypogaea L.)

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Abstract

Late leaf spot (LLS) is the major foliar disease, which often occur with rust and cause severe yield losses worldwide in peanut (Arachis hypogaea L.). In order to map and introgress the resistance alleles for LLS into an elite cultivar ICGS 76, the advanced backcross approach was used for identification of quantitative trait locus (QTL). The synthetic amphidiploid ISATGR 278–18 (A. duranensis ICG 8138 × A. batizocoi ICG 13160) which is known to harbor resistance alleles for foliar diseases was used as donor parent to generate AB-QTL population comprising of 164 introgression lines (ILs). A linkage map with 114 microsatellite markers was developed spanning 746.15 cM distance with an average inter-marker distance of 6.55 cM. QTL analysis yielded 14 main-effect QTLs (mQTLs) (explaining up to 38.58% PVE) for LLS and 10 mQTLs (up to 10.99% PVE) for rust. In addition, four epistatic QTLs (eQTLs) (explaining up to 4.97% PVE) were observed for foliar diseases. Furthermore, seven mQTLs and 16 eQTLs were observed for agronomic traits. Several ILs showed high resistance to LLS with agronomic attributes from recurrent parent. This study reports the utilization of the untapped resistance source for LLS from A. batizocoi that was not extensively used earlier in peanut crop improvement.

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Abbreviations

QTL:

Quantitative trait loci

ILs:

Introgression lines

PLHT:

Plant height

LL:

leaf length

LW:

leaf width

NOPB:

number of primary branches

NOSB:

number of secondary branches

SP:

shelling percent

TPW:

total pod weight

TW:

test weight

Rust80:

rust scored after 80 days after sowing

Rust90:

rust scored 90 days after sowing

LLS80:

late leaf spot scored 80 days after sowing

LLS90:

late leaf spot scored 90 days after sowing

PCV:

Phenotypic coefficient of variance,

GCV:

Genotypic coefficient of variance

h2bs. %:

Broad sense heritability

GAM:

Genetic advance over mean

AB-QTL:

Advanced backcross quantitative trait loci

PVE%:

phenotypic variance explained in percent

DAS:

days after sowing

Kharif:

seasons are rainy seasons

Summer:

is post-rainy season

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Kumari, V., Gowda, M.V.C. & Yeri, S.B. Utilization of Advanced Backcross Population Derived from Synthetic Amphidiploid for Dissecting Resistance to Late Leaf Spot in Peanut (Arachis hypogaea L.). Tropical Plant Biol. 13, 50–61 (2020). https://doi.org/10.1007/s12042-019-09246-y

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Keywords

  • Advanced backcross
  • Genetic linkage map
  • Peanut
  • Synthetic amphidiploids
  • QTL