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Tropical Plant Biology

, Volume 6, Issue 2–3, pp 69–84 | Cite as

Thermal-Stable Proteins of Fruit of Long-Living Sacred Lotus Nelumbo nucifera Gaertn var. China Antique

  • J. Shen-MillerEmail author
  • Petra Lindner
  • Yongming Xie
  • Sarah Villa
  • Kerry Wooding
  • Steven G. Clarke
  • Rachel R. O. Loo
  • Joseph A. Loo
Article

Abstract

Single-seeded fruit of the sacred lotus Nelumbo nucifera Gaertn var. China Antique from NE China have been shown to remain viable for as long as ~1,300 years, determined by direct radiocarbon-dating, and to have a germination rate of 84 %. The pericarp, a fruit tissue that encloses the single seeds of Nelumbo, is one of the major factors contributing to fruit longevity. Proteins that are heat stable and have a protective function are equally important to such centuries-long seed viability. We document proteins of Nelumbo fruit that are able to withstand heating, 32 % of which remained soluble in the 110 °C-treated embryo axis of a 549-year-old fruit and 76 % retained fluidity in its cotyledons. The genome of Nelumbo has recently been published and annotated. The amino-acid sequences of 11 “thermal proteins” (soluble at 100 °C) of modern Nelumbo embryo axes and cotyledons, identified by mass spectrometry, Western blot and bioassay, are assembled and aligned with those of an archaeal hyperthermophile Methancaldococcus jannaschii (“Mj,” an anaerobic methanogen having a growth optimum of 85 °C) and with those of five mesophile angiosperms. These thermal proteins have roles in protection and repair under stress. More than half (55 %) of the durable Nelumbo thermal proteins are present in the archaean Mj, indicating their ancient history. One Nelumbo protein-repair enzyme exhibits activity at 100 °C, having a heat-tolerance higher than the comparable enzyme of Arabidopsis. A list of 30 sequenced but unassembled thermal proteins of Nelumbo is appended.

Keywords

Nelumbo nucifera China antique Heat-soluble (100 °C) Proteins Hyperthermophile-Mesophile protein-alignments Stress-and-repair thermoproteins 

Abbreviations

AdoMet

S-adenosyl-L-methionine

CPN20/60

Chaperonine20/60

CuZn-SOD

Copper-zinc superoxide dismutase

1-CysPRX

1-Cys peroxiredoxin

ENO1

Enolase1

EF-1α

Elongation factor-1α

EST

Expressed sequence tag

HSP80

Heat-shock protein 80

LC-MS-MS

Liquid-chromatography tandem mass-spectrometry

Mj

Methancaldococcus jannaschii

PIMT

Protein L-isoaspartyl methyltransferase

ROS

Reactive oxygen-species

Notes

Acknowledgments

We thank the reviewers for valuable suggestions, C. Haas-Blaby for bioinformatics and protein alignments, J.W. Schopf for constructive comments, J. Lowenson for helpful discussions, and H. Nguyen for compilation assistance of the MS data. For antisera, we are grateful to P. Viitanen for CPN20 and CPN60, T. Close for dehydrin, and B. Downie and D. Martin for PIMT1. Work in SGC laboratory is supported by NIH grant GM206020. We also thank K.O. Stetter whose seminal research on hyperthermophiles stimulated this ‘hot protein’ study of Nelumbo enzymes.

Supplementary material

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • J. Shen-Miller
    • 1
    Email author
  • Petra Lindner
    • 2
  • Yongming Xie
    • 3
  • Sarah Villa
    • 4
  • Kerry Wooding
    • 3
  • Steven G. Clarke
    • 4
  • Rachel R. O. Loo
    • 3
  • Joseph A. Loo
    • 3
  1. 1.IGPP Center for the Study of Evolution and the Origin of Life, Department of Ecology and Evolutionary BiologyUniversity of California, Los AngelesLos AngelesUSA
  2. 2.Lehrstuhl MikrobiologieRegensburg UniversityRegensburgGermany
  3. 3.Department of Chemistry and BiochemistryUniversity of California, Los AngelesLos AngelesUSA
  4. 4.Department of Chemistry and BiochemistryUniversity of California, Los AngelesLos AngelesUSA

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