Tropical Plant Biology

, Volume 5, Issue 3, pp 218–232

Dehydrins Are Highly Expressed in Water-Stressed Plants of Two Coffee Species



Drought is the main limiting factor for coffee productivity. In this study, we evaluated the relationship between dehydrins (DHN) and water status in Coffea arabica cvs. Catuaí and Mundo Novo, C. canephora cv. Apoatã, and a graft of Mundo Novo shoot on Apoatã root. The plants were control stressed to achieve a water potential (ψw) of approximately −2.15 ± 0.05 MPa at predawn (6:00 am). Measurements of ψw on the preceding day (at 12:00 noon) and at predawn showed that the Arabicas had greater losses of shoot and root dry mass. Additionaly, proline increased in roots and leaves of all plants, indicating stress establishment. Two DHN unigenes in C. arabica (CaDHN1 and CaDHN3) and one in C. racemosa (CrDHN1) were identified from an expressed sequence tag database with greater than 95 % identity. Three DHN genes named CcDH1, CcDH2, and CcDH3 isolated in previous work from coffee fruits of C. canephora were analysed in this study too. Transcripts of DHN1, DHN2, and DHN3 accumulated in roots and leaves of stressed plants and also in cell suspension cultures of Catuaí stressed with PEG-8000. While DHN1 and DHN3 exhibited basal expression levels, DHN2 was exclusively expressed in stressed plants. Although DHN unigenes were induced by water stress, the expression pattern of each unigene was spatially (leaves and roots) and temporally (distinct stress levels) differentiated, as was the intensity of the responses among the Arabicas, Apoatã, and MN/Apoatã plants. Our results suggest a strong relationship between DHN expression and water stress in coffee.


Coffea arabica Coffea canephora Dehydrins Drought tolerance Root system 


  1. Alfonsi EL, Fahl JI, Carelli MLC, Fazuoli LC (2005) Crescimento, fotossíntese e composição mineral em genótipos de Coffea com potencial para utilização como porta enxerto. Bragantia 64:1–13CrossRefGoogle Scholar
  2. Allagulova CR, Gimalov FR, Shakirova FM, Vakhitov VA (2003) The plant dehydrins: structure and putative functions. Biochemistry 68:945–951PubMedGoogle Scholar
  3. Baker J, Steele C, Dure L (1988) Sequence and characterization of 6 Lea proteins and their genes from cotton. Plant Mol Biol 11:277–291CrossRefGoogle Scholar
  4. Barsalobres-Cavallari C, Severino F, Maluf M, Maia I (2009) Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions. BMC Mol Biol 10:1PubMedCrossRefGoogle Scholar
  5. Bassett CL, Wisniewski ME, Artlip TS, Richart G, Norelli JL, Farrell RE Jr (2009) Comparative expression and transcript initiation of three peach dehydrin genes. Planta 230:107–118PubMedCrossRefGoogle Scholar
  6. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  7. Beck EH, Fettig S, Knake C, Hartig K, Bhattarai T (2007) Specific and unspecific responses of plants to cold and drought stress. J Biosci 32:501–510PubMedCrossRefGoogle Scholar
  8. Berthaud J, Charrier A (1988) Genetic resources of Coffea. In: Clarke RJ, Macrae R (eds) Coffee: agronomy, vol IV. Elsevier Applied Science, London, pp 1–42Google Scholar
  9. Carr MKV (2001) The water relations and irrigation requirements of coffee. Expl Agric 37:1–36CrossRefGoogle Scholar
  10. Cellier F, Conéjéro G, Breitler JC, Casse F (1998) Molecular and physiological responses to water deficit in drought-tolerant and drought-sensitive lines of sunflower. Plant Physiol 116:319–328PubMedCrossRefGoogle Scholar
  11. Chevalier A (1942) Les caféiers du globe. Fascicule II. Iconographie des caféiers sauvages et cultivés, Enciclopedie Bioloique, vol XXII, ParisGoogle Scholar
  12. Choi D-W, Zhu B, Close TJ (1999) The barley (Hordeum vulgare L.) dehydrin multigene family: sequences, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv. Dicktoo. Theor Appl Genet 98:1234–1247CrossRefGoogle Scholar
  13. Choi DW, Rodriguez EM, Close TJ (2002) Barley Cbf3 gene identification, expression pattern, and map location. Plant Physiol 129:1781–1787PubMedCrossRefGoogle Scholar
  14. Close TJ (1996) Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plant 97:795–803CrossRefGoogle Scholar
  15. Close TJ (1997) Dehydrins: a commonalty in the response of plants to dehydration and low temperature. Physiol Plant 100:291–296CrossRefGoogle Scholar
  16. DaMatta FM, Ramalho JDC (2006) Impacts of drought and temperature stress on coffee physiology and production: a review. Braz J Plant Physiol 18:55–81CrossRefGoogle Scholar
  17. DaMatta FM, Maestri M, Barros RS, Regazzi AJ (1993) Water relations of coffee leaves (Coffea arabica and C. canephora) in response to drought. J Hort Sci 68:741–746Google Scholar
  18. DaMatta FM, Maestri M, Barros RS (1997) Photosynthetic performance of two coffee species under drought. Photosynthetica 34:257–264CrossRefGoogle Scholar
  19. DaMatta FM, Chaves ARM, Pinheiro HA, Ducatti C, Loureiro ME (2003) Drought tolerance of two field-grown clones of Coffea canephora. Plant Sci 164:111–117CrossRefGoogle Scholar
  20. Davis AP, Govaerts R, Bridson DM, Stoffelen P (2006) An annotated taxonomic conspectus of genus Coffea (Rubiaceae). Bot J Linn Soc 152:465–512CrossRefGoogle Scholar
  21. Dias PC, Araujo WL, Moraes GABK, Barros RS, DaMatta FM (2007) Morphological and physiological responses of two coffee progenies to soil water availability. J Plant Physiol 164:1639–1647PubMedCrossRefGoogle Scholar
  22. Dubouzet JG, Sakuma Y, Ito Y, Kasuga M, Dubouzet EG, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J 33:751–763PubMedCrossRefGoogle Scholar
  23. Dure L (1993) A repeating 11-mer amino acid motif and plant desiccation. Plant J 3:363–369PubMedCrossRefGoogle Scholar
  24. Fahl JL, Carelli MLC, Costa WM, Novo MCSS (1998) Enxertia de Coffea arabica sobre progênies de C. canephora e de C. congensis no crescimento, nutrição mineral e produção. Bragantia 57:297–312CrossRefGoogle Scholar
  25. Fahl JI, Carelli MLC, Menezes HC, Gallo PB, Trivelin PCO (2001) Gas exchange, growth, yield and beverage quality of Coffea arabica cultivars grafted on to C. canephora and C. congensis. Exp Agric 37:241–252CrossRefGoogle Scholar
  26. Fernandez M, Águila SV, Arora R, Chen K (2012) Isolation and characterization of three cold acclimation-responsive dehydrin genes from Eucalyptus globulus. Tree Genet Gen 8:149–162CrossRefGoogle Scholar
  27. Geromel C, Ferreira LP, Bonatelli ML, Bottcher A, Pot D, Pereira LFP, Leroy T, Vieira LGE, Mazzafera P (2008) Sucrose metabolism during fruit development of Coffea racemosa. Ann Appl Biol 152:179–187CrossRefGoogle Scholar
  28. Guerreiro Filho O (1992) Coffea racemosa Lour. Une revue. Café Cacao Thé 26:171–186Google Scholar
  29. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  30. Hinniger C, Caillet V, Michoux F, Ben Amor M, Tanksley S, Lin CW, McCarthy J (2006) Isolation and characterization of cDNA encoding three dehydrins expressed during Coffea canephora (Robusta) grain development. Ann Bot 97:755–765PubMedCrossRefGoogle Scholar
  31. Ismail AM, Hall AE, Close TJ (1999) Purification and partial characterization of a dehydrin involved in chilling tolerance during seedling emergence of cowpea. Plant Physiol 120:237–244PubMedCrossRefGoogle Scholar
  32. Iwasaki T, Yamaguchi-Shinozaki K, Shinozaki K (1995) Identification of a cis-regulatory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis. Mol Gen Genet 247:391–398PubMedCrossRefGoogle Scholar
  33. Kramer D, Breitenstein B, Kleinwächter M, Selmar D (2010) Stress metabolism in green coffee beans (Coffea arabica L.): Expression of dehydrins and accumulation of GABA during drying. Plant Cell Physiol 51:546–553PubMedCrossRefGoogle Scholar
  34. Lin CW, Mueller LA, Mc Carthy J, Crouzillat D, Petiard V, Tanksley SD (2005) Coffee and tomato share common gene repertoires as revealed by deep sequencing of seed and cherry transcripts. Theor Appl Gen 112:114–130CrossRefGoogle Scholar
  35. Lopez CG, Banowetz GM, Peterson CJ, Kronstad WE (2003) Dehydrin expression and drought tolerance in seven wheat cultivars. Crop Sci 43:577–582CrossRefGoogle Scholar
  36. Maestri M, DaMatta FM, Regazzi AJ, Barros RS (1995) Accumulation of proline and quaternary ammonium compounds in mature leaves of water stressed coffee plants (Coffea arabica and C. canephora). J Hort Sci 70:229–233Google Scholar
  37. Marraccini P, Vinecky F, Alves GSC, Ramos HJO, Elbelt S, Vieira NG, Carneiro FA, Sujii PS, Alekcevetch JC, Silva VA, DaMatta FM, Ferrão MAG, Leroy T, Pot D, Vieira LGE, Silva FR, Andrade AC (2012) Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. J Exp Bot. doi:10.1093/jxb/ers103
  38. Mazzafera P, Teixeira JPF (1989) Prolina em cafeeiros submetidos a déficit hídrico. Turrialba 39:305–313Google Scholar
  39. Medina Filho HP, Carvalho A, Monaco LC (1977) Germoplasma de Coffea racemosa e seu potencial no melhoramento do cafeeiro. Bragantia XLIII-XLVIGoogle Scholar
  40. Meinzer FC, Grantz DA, Goldstein G, Saliendra NZ (1990) Water relations and maintenance of gas exchange in coffee cultivars grown in a drying soil. Plant Physiol 94:1781–1787PubMedCrossRefGoogle Scholar
  41. Mondego J, Vidal R, Carazzolle M, Tokuda E, Parizzi L, Costa G, Pereira L, Andrade A, Colombo C, Vieira L, Pereira G, Consortium BCGP (2011) An EST-based analysis identifies new genes and reveals distinctive gene expression features of Coffea arabica and Coffea canephora. BMC Plant Biol 11:30PubMedCrossRefGoogle Scholar
  42. Moraes MV, Franco CM (1973) Método expedito para enxertia em café. Boletim do Instituto Brasileiro de Café, 8pGoogle Scholar
  43. Neuenschwander B, Baumann TW (1992) A novel type of somatic embryogenesis in Coffea arabica. Plant Cell Rep 10:608–612CrossRefGoogle Scholar
  44. Nylander M, Svensson J, Palva ET, Welin BV (2001) Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana. Plant Mol Biol 45:263–279PubMedCrossRefGoogle Scholar
  45. Passioura JB (1997) Drought and drought tolerance. In: Belhassen I (ed) Drought tolerance in higher plants: genetical, physiological, and molecular biological analysis. Kluwer Academic, Dordrecht, pp 1–7Google Scholar
  46. Pinheiro HA, DaMatta FM, Chaves ARM, Loureiro ME, Ducatti C (2005) Drought tolerance is associated with rooting depth and stomatal control of water use in clones of Coffea canephora. Ann Bot 96:101–108PubMedCrossRefGoogle Scholar
  47. Praxedes SC, DaMatta FM, Loureiro ME, Ferrão MAG, Cordeiro AT (2006) Effects of long-term soil drought on photosynthesis and carbohydrate metabolism in mature robusta coffee (Coffea canephora Pierre var. kouillou) leaves. Environ Exp Bot 56:263–273CrossRefGoogle Scholar
  48. Rena AB, Barros RS, Maestri M, Sondahl MR (1994) Coffee. In: Schaffer B, Andersen PC (eds) Handbook of environmental physiology of fruit crops: subtropical and tropical crops, vol 2. CRC, Boca Raton, pp 101–122Google Scholar
  49. Rezaian MA, Krake LR (1987) Nucleic acid extraction and virus detection in grapevine. J Virol Meth 17:277–285CrossRefGoogle Scholar
  50. Rezende AM, Rosado PL (2004) A informação no mercado de café. In: Zambolim L (ed) Produção integrada de café. Universidade Federal de Viçosa, Viçosa, pp 1–46Google Scholar
  51. Rodríguez EM, Svensson JT, Malatrasi M, Choi DW, Close TJ (2005) Barley Dhn13 encodes a KS-type dehydrin with constitutive and stress responsive expression. Theor Appl Gen 110:852–858CrossRefGoogle Scholar
  52. Rorat T, Grygorowicz WJ, Irzykowski W, Rey P (2004) Expression of KS-type dehydrins is primarily regulated by factors related to organ type and leaf developmental stage during vegetative growth. Planta 218:878–885PubMedCrossRefGoogle Scholar
  53. Rorat T, Szabala B, Grygorowicz W, Wojtowicz B, Yin Z, Rey P (2006) Expression of SK3-type dehydrin in transporting organs is associated with cold acclimation in Solanum species. Planta 224:205–221PubMedCrossRefGoogle Scholar
  54. Sartor RM, Mazzafera P (2000) Caffeine formation by suspension cultures of Coffea dewevrei. Braz Arch Biol Technol 43:61–69CrossRefGoogle Scholar
  55. Silva EA, Mazzafera P (2008) Influences of temperature and water in the coffee culture. Am J Plant Sci Biotechnol 2:32–41Google Scholar
  56. Silva VA, Antunes WC, Guimarães BLS, Paiva RMC, Silva VF, Ferrão MAG, DaMatta FM, Loureiro ME (2010) Resposta fisiológica de clone de café Conilon sensível à deficiência hídrica enxertado em porta-enxerto tolerante. Pesq Agropec Bras 45:457–464CrossRefGoogle Scholar
  57. Vieira LGE et al (2006) Brazilian coffee genome project: an EST-based genomic resource. Braz J Plant Physiol 18:95–108CrossRefGoogle Scholar
  58. Xiaoqiu H (1992) A contig assembly program based on sensitive detection of fragment overlaps. Genomics 14:18–25CrossRefGoogle Scholar
  59. Yang L, Zheng B, Mao C, Qi X, Liu F, Wu P (2004) Analysis of transcripts that are differentially expressed in three sectors of the rice root system under water deficit. Mol Genet Genom 272:433–442CrossRefGoogle Scholar
  60. Zhu B, Choi DW, Fenton R, Close TJ (2000) Expression of the barley dehydrin multigene family and the development of freezing tolerance. Mol Gen Genet 264:145–153PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Plant Biology, Institute of BiologyState University of CampinasCampinasBrazil

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