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Tropical Plant Biology

, Volume 3, Issue 4, pp 204–218 | Cite as

QTL Analysis and Effect of the fin Locus on Tropical Adaptation in an Inter-Gene Pool Common Bean Population

  • M. Carolina Chavarro
  • Matthew W. Blair
Article

Abstract

Common bean architecture is in part determined by the determinacy gene fin which can affect yield potential and adaptation to various environments and has many known effects in temperate regions. Tropical adaptation, meanwhile, requires adaptation to rainy and dry seasons where heat and drought stress are major problems. The goal of this research was to determine the effect of the fin gene on plants grown under heat, drought and non-stress conditions in the tropics and to identify quantitative trait loci (QTL) for architectural, phenological and yield traits related to fin in an inter-genepool population derived from a cross between an indeterminate Mesoamerican genotype with erect architecture (A55) and a determinate Andean genotype with heat tolerance (G122). The population was evaluated in four experiments conducted in a tropical location across two rainy seasons and across dry season drought stress and dry season irrigated treatments. A total of 71 SSR loci and 245 AFLP, RAPD, seed protein or phenotypic markers were integrated together into a genetic map with a total distance of 982.8 cM. A total of 36 QTL were identified based on the evaluation of six phenotypic variables differentiating the parents. The A55 parent was high yielding under all conditions; while G122 was confirmed to be resistant to high temperatures, but not to drought. Some QTL for yield were associated with erect growth alleles inherited from the indeterminate parent and were located on linkage group b01 near the fin locus while other QTL for seed weight were found across the genome.

Keywords

Abiotic stress Heat tolerance Drought resistance Quantitative trait loci Phaseolus vulgaris 

Abbreviations

AFLP

Amplified fragment length polymorphisms

cM

Centimorgan

DAP

Days After Planting

Df

Days to Flowering

Dm

Days to Physiological Maturity

DS-I

Drought Stress Irrigated

DS-DS

Drought Stress Dry Season

GLM

Generalized Linear Model

LOD

Likelihood of Odds Ratio

QTL

Quantitative Trait Loci

RAPD

Random Amplified Polymorphic DNA

RFLP

Restriction Fragment Length Polymorphism

RIL

Recombinant Inbred Line

RS1

First Rainy Seasons

RS2

Second Rainy Seasons

SCAR

Sequence-Characterized Amplified Region

SSR

Single Sequence Repeats

SSD

Single-Seed Descent

Sw

100-Seed Weight

Notes

Acknowledgments

We thank Ingrid Schuler for her suggestions for this work, Elizabeth Paez for editorial help, Héctor Fabio Buendía for technical assistance, Bill Johnson and Paul Gepts for mapping results, and Steve Magnusson and Phil Miklas for germplasm development and supply. Finally, Agobardo Hoyos helped with statistical analyses.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.International Center for Tropical Agriculture (CIAT)CaliColombia

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