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Tropical Plant Biology

, Volume 3, Issue 3, pp 166–170 | Cite as

Integration of Genetic and Cytological Maps and Development of a Pachytene Chromosome-based Karyotype in Papaya

  • Wenli Zhang
  • Ching Man Wai
  • Ray Ming
  • Qingyi Yu
  • Jiming Jiang
Article

Abstract

A significant amount of genetic and genomic resources have been developed in papaya (Carica papaya, \( {\hbox{2n = 2}} \times { = 18} \)), including genetic linkage maps consisting of nine major and three minor linkage groups. However, the 12 genetic linkage groups have not been integrated with the nine chromosomes of papaya. Bacterial artificial chromosome (BAC) clones associated with each linkage group were recently isolated. These linkage group-specific BACs were mapped to meiotic pachytene chromosomes of papaya using fluorescence in situ hybridization (FISH). The FISH mapping results integrated the 12 linkage groups into the nine papaya chromosomes. We developed a pachytene chromosome-based high resolution karyotype for the hermaphrodite plant genome of papaya cultivar SunUp. The chromosomal distribution of heterochromatin in the papaya genome is provided in the karyotype with the X chromosome representing the most euchromatic chromosome in the papaya genome. FISH mapping also revealed a significant amplification of sequences related to the 5S ribosomal RNA genes, which was detected in the male-specific region of the Y chromosome, but not in the corresponding region in the X chromosome.

Keywords

Carica Chromosome map FISH Karyotype 

Abbreviations

BAC

Bacterial artificial chromosome

DAPI

4′ 6-diamidino-2-phenylindole

FISH

Fluorescence in situ hybridization

LG

Linkage group

MSY

Male-specific region of the Y chromosome

SSR

Simple sequence repeat

Notes

Acknowledgments

This research was supported by grant DBI-0553417 from the National Science Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Wenli Zhang
    • 1
  • Ching Man Wai
    • 2
  • Ray Ming
    • 3
  • Qingyi Yu
    • 2
    • 4
  • Jiming Jiang
    • 1
  1. 1.Department of HorticultureUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Hawaii Agriculture Research CenterAieaUSA
  3. 3.Department of Plant BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  4. 4.Texas AgriLife Research Center at WeslacoTexas A&M UniversityWeslacoUSA

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