Journal of Genetics

, 98:113 | Cite as

Genetics of novel brown planthopper Nilaparvata lugens (Stål) resistance genes in derived introgression lines from the interspecific cross O. sativa var. Swarna × O. nivara

  • S. Akanksha
  • V. Jhansi Lakshmi
  • Arun Kumar Singh
  • Yamini Deepthi
  • P. M. Chirutkar
  • Ramdeen
  • Divya Balakrishnan
  • N. Sarla
  • S. K. Mangrauthia
  • T. RamEmail author
Research Article


The brown planthopper (BPH) Nilaparvata lugens (Stål) (Homoptera: Delphacidae) is considered a threat to rice (Oryza sativa ssp.) crop in many parts of the world including India. Among the BPH-resistance (R) genes so far reported in rice, most of them are ineffective against BPH biotype 4 predominant in the Indian sub-continent. In this study, we show the introgression line RPBio4918-230S was identified as BPH resistant after five years of rigorous screening at seedling stage and two years at tillering and reproductive stages. The inheritance of resistance indicated that two recessive genes are involved at seedling and reproductive stages. The allelic relation with known genes using linked reported markers suggested that the genes present in RPBio4918-230S are different. We report here the genetics of the two newly introgressed BPH resistance genes from O. nivara in the background of Swarna which are effective at all the important growth stages. The genes have been tentatively named as bph39(t) and bph40(t). The honeydew area (feeding rate) and days to wilt parameters observed at 30 days after sowing in BC1F3 indicated that newly introgressed genes have both antibiosis and tolerance mechanisms for resistance. The BPH resistance genes identified in this study would facilitate the breeding of broad spectrum and durable resistance in rice against BPH biotype 4.


brown planthopper novel gene introgression reproductive stage resistance rice Nilaparvata lugens Oryza nivara 



We thank Dr P. V. Satyanarayana, APRRI and RARS Maruteru, India, for providing facilities for conducting field trials. We thank Director, IIRR, Hyderabad and faculty of Osmania University, Hyderabad for the support in carrying out this research programme.

Supplementary material

12041_2019_1158_MOESM1_ESM.doc (4.4 mb)
Supplementary material 1 (DOC 4462 kb)


  1. Akanksha I., Jhansilakshmi V., Chirutkar P. M., Subba Rao L. V., Sarla N. and Ram T. 2017 Identification of new source for BPH resistance from rice germplasm and introgression lines derived from O. nivara. Oryza 54, 272–275.Google Scholar
  2. Alagar M. and Suresh S. 2007 Population build-up of brown planthopper, Nilaparvata lugens on selected rice genotypes. Ind. J. Plant Prot. 35, 32–35.Google Scholar
  3. Alam S. N. and Cohen M. B. 1998 Detection and analysis of QTLs for resistance to the brown planthopper, Nilaparvata lugens, in a double-haploid rice population. Theor. Appl. Genet. 97, 1370–1379.Google Scholar
  4. Bae S. H. and Pathak M. D. 1970 Life History of Nilaparvata lugens (Homoptera: Delphacidae) and susceptibility of rice varieties to its attacks. Ann. Entomol. Soc. Am. 63, 149–155.Google Scholar
  5. Balachiranjeevi C. H., Prahalada G. D., Mahender A., Jamaloddin M., Sevilla M. A. L., Marfori-Nazarea C. M. et al. 2019 Identification of a novel locus, BPH38(t), conferring resistance to brown planthopper (Nilaparvata lugens Stal.) using early backcross population in rice (Oryza sativa L.). Euphytica 215, 185.Google Scholar
  6. Chen X., Temnykh S., Xu Y., Cho Y. G. and McCouch S. R. 1997 Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theor. Appl. Genet. 95, 553–567.Google Scholar
  7. Du B., Zhang W., Liu B., Hu J., Wei Z., Shi Z. et al. 2009 Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice. Proc. Natl. Acad. Sci. USA 106, 22163– 22168.PubMedGoogle Scholar
  8. Fujita D., Kohli A. and Horgan F. G. 2013 Rice resistance to planthoppers and leafhoppers. Crit. Rev. Plant Sci. 32, 162–191.Google Scholar
  9. Geethanjali S., Kadirvel P., Gunathilagaraj K. and Maheswaran M. 2009 Detection of quantitative trait loci (QTL) associated with resistance to whitebacked planthopper (Sogatella furcifera) in rice (Oryza sativa). Plant Breed. 128, 130–136.Google Scholar
  10. Hao P., Liu C., Wang Y., Chen R., Tang M., Du B. et al. 2008 Herbivore-induced callose deposition on the sieve plates of rice: an important mechanism of host resistance. Plant Physiol. 146, 1810–1820.PubMedPubMedCentralGoogle Scholar
  11. Harushima Y., Nakagahra M., Yano M., Sasaki T. and Kurata N. 2002 Diverse variation of reproductive barriers in three intraspecific rice crosses. Genetics 160, 313–322.PubMedPubMedCentralGoogle Scholar
  12. Heinrichs E. A., Medrano F. G. and Rapusas H. R. 1985 Genetic evaluation for insect resistance in rice. International Rice Research Institute, Los Banos, Philippines.Google Scholar
  13. Heong K. L. 2009 Are planthopper problems due to breakdown in ecosystem services? In Planthoppers – new threats to the sustainability of intensive rice production systems in Asia (ed. K. L. Heong and B. Hardy). International Rice Research Institute, pp. 221–232. Los Banos.Google Scholar
  14. Hu Jie., Cong Xiao and Yuqing He. 2016 Recent progress on the genetics and molecular breeding of brown planthopper resistance in rice. Rice 9, 30.PubMedPubMedCentralGoogle Scholar
  15. Huang Z., He G., Shu L., Li X. and Zhang Q. 2001 Identification and mapping of two brown planthopper resistance genes in rice. Theor. Appl. Genet. 102, 929–934.Google Scholar
  16. Jairin J., Teangdeerith S., Leelagud P., Phengrat K., Vanavichit A. and Toojinda T. 2007 Physical mapping of Bph3, a brown planthopper resistance locus in rice. Maejo Int. J. Sci. Tech. 1, 166–177.Google Scholar
  17. Jena K. K., Hechanova S. L., Verdeprado H., Prahalada G. D. and Kim S. R. 2017 Development of 25 near-isogenic lines (NILs) with ten BPH resistance genes in rice (Oryza sativa L.): production, resistance spectrum, and molecular analysis. Theor. Appl. Genet. 130, 2345–2360.PubMedGoogle Scholar
  18. Kar S., Verulkar S. B., Verma S. and Pali V. 2011 Identification of QTLs for brown planthopper (Nilaparvata lugens (Stål.)) resistance in RIL mapping population of rice (Oryza sativa L.). Plant Arch. 11, 687–692.Google Scholar
  19. Kim S., UnSang Y. and JaeKeun S. 2004 Classification of cultivars resistant to brown planthopper using DNA markers in rice. Korean J. Breed. 36, 290–294.Google Scholar
  20. Kumar K., Sarao P. S, Bhatia D., Neelam K., Kaur A, Mangat G. S. et al. 2018 High-resolution genetic mapping of a novel brown planthopper resistance locus, Bph34 in Oryza sativa L. X Oryza nivara (Sharma & Shastry) derived interspecific F2 population. Theor. Appl. Genet. 131, 1163–1171.PubMedGoogle Scholar
  21. Ling K. C., Tiongco E. R. and Aguiero V. M. 1978 Rice ragged stunt, a new virus disease. Plant Dis. Rep. 62, 701–705.Google Scholar
  22. Liu Y., Su C. Jiang L., He J., Wu H., Peng C. and Wan J. M. 2009 The distribution and identification of brown planthopper resistance genes in rice. Hereditas 146, 67–73.PubMedGoogle Scholar
  23. Liu Y., Wu H., Chen H., Liu Y., He J., Kang H. et al. 2015 A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insect resistance in rice. Nat. Biotechnol. 33, 301–305.PubMedGoogle Scholar
  24. Lv W., Du B., Shangguan X., Zhao Y., Pan Y., Zhu L. et al. 2014 BAC and RNA sequencing reveal the brown planthopper resistance gene Bph15 in a recombination cold spot that mediates a unique defence mechanism. BMC Genomics 15, 674.PubMedPubMedCentralGoogle Scholar
  25. Myint K. K. M., Yasui H., Takagi M. and Matsumura M. 2009a Virulence of long-term laboratory populations of the brown planthopper, Nilaparvata lugens (Stål), and whitebacked planthopper, Sogatella furcifera (Horvath) (Homoptera: Delphacidae), on rice differential varieties. Appl. Entomol. Zool. 44, 149–153.Google Scholar
  26. Myint K. K. M., Matsumura M., Takagi M. and Yasui H. 2009b Demographic parameters of long-term laboratory strains of the brown planthopper, Nilaparvata lugens (Stål) (Homoptera: Delphacidae) on resistance genes bph20(t) and Bph21(t) in rice. J. Fac. Agr. Kyushu U. 54, 159–164.Google Scholar
  27. Naik S. B., Divya D., Sahu N., Sundaram R. M., Sarao P. S., Singh K. et al 2018 A new gene Bph33(t) conferring resistance to brown planthopper (BPH), Nilaparvata lugens (Stål) in rice line RP2068-18-3-5. Euphytica 214, 53.Google Scholar
  28. Normile D. 2008 Reinventing rice to feed the world. Science 321, 330–333.Google Scholar
  29. Qiu Y. F., Guo J. P., Jing S. L., Zhu L. L. and He G. C. 2010 High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds. Theor. Appl. Genet. 121, 1601–1611.PubMedGoogle Scholar
  30. Qiu Y. F., Guo J. P., Jing S. Q., Zhu L. L. and He G. C. 2014 Fine mapping of the rice brown planthopper resistance gene Bph7 and characterization of its resistance in the 93-11 background. Euphytica 198, 369–379.Google Scholar
  31. Ramdeen, Ramesh K., Padmavathi G., Viraktamath B. C. and Ram T. 2017 Mapping of brown planthopper [Nilaparvata lugens (Stål)] resistance gene (bph5) in rice (Oryza sativa L.). Euphytica 213, 35.Google Scholar
  32. Ram T., Deen R., Gautam, S. K., Ramesh K., Rao Y. K. and Brar D. S. 2010 Identification of new genes for brown planthopper resistance in rice introgressed from O. glaberrima and O. minuta. Rice Genet. Newslett. 25, 67–69.Google Scholar
  33. Ramesh K., Padmavathi G., Deen R., Pandey M. K., Lakshmi V. J. and Bentur J. S. 2014 Whitebacked planthopper Sogatella furcifera (Horvath) (Homoptera: Delphacidae) resistance in rice variety Sinna Sivappu. Euphytica 200, 139–148.Google Scholar
  34. Ren J., Gao F., Wu X., Lu X., Zeng L., Lv J. et al. 2016 Bph32, a novel gene encoding an unknown SCR domain-containing protein, confers resistance against the brown planthopper in rice. Sci. Rep. 23, 37645.Google Scholar
  35. Reissing W. H., Heinrichs E. A. and Valencia S. L. 1982 Insecticide-induced resurgence of the brown planthopper, Nilaparvata lugens, on rice varieties with different levels of resistance. Environ. Entomol. 11, 165–168.Google Scholar
  36. Saghai Maroof M. A., Biyashav R. M., Yang G. P., Zhang Q. and Allard R. W. 1994 Extraordinary polymorphic microsatellite DNA in barley: species diversity, chromosomal locations and population dynamics, Proc. Nat. Acad. Sci. USA 91, 5466–5470.PubMedGoogle Scholar
  37. Sarao P. S. and Mangat G. S. 2014 Manage rice insect-pests to get higher crop yield. Prog. Farming 50, 4–6.Google Scholar
  38. Sarao P. S. and Bentur J. S. 2016 Antixenosis and tolerance of rice genotypes against brown planthopper. Rice Sci. 23, 96–103.Google Scholar
  39. Sogawa K. 1994 Feeding behaviour and damage mechanism of the rice planthoppers. In Analysis of damage mechanisms by pests and diseases and their effects on rice yield (ed. A. Elings and E. G. Rubia), SARP research proceedings, pp. 143–154. Wageningen, The Netherlands.Google Scholar
  40. Song X. L., Qiang S., Liu L. L., Xu Y. H. and Liu Y. L. 2002 Gene flow of pollen cross between Oryza officinalis wall and transgenetic rice with bar gene. J. Nanjing Agr. Univ. 25, 5–8.Google Scholar
  41. Tamura Y., Hattori M., Yoshioka H., Yoshioka M., Takahashi A., Wu J. et al. 2014 Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52. Sci. Rep. 4, 5872.Google Scholar
  42. Wang Y., Cao L., Zhang Y., Cao C., Liu F., Huang F. et al. 2015 Map-based cloning and characterization of BPH29, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice. J. Exp. Bot. 66, 6035–6045.PubMedPubMedCentralGoogle Scholar
  43. Watanabe T. and Kitagawa H. 2000. Photosynthesis and translocation of assimilates in rice plants following phloem feeding by the planthopper Nilaparvata lugens (Homoptera: Delphacidae). J. Econ. Entomol. 93, 1192–1198.PubMedGoogle Scholar
  44. Wu H., Liu Y., He J., Liu Y., Jiang L., Liu L. et al. 2014 Fine mapping of brown planthopper (Nilaparvata lugens Stål) resistance gene Bph 28(t) in rice (Oryza sativa L.). Mol. Breed. 33, 909–918.Google Scholar
  45. Xu Y., Zhu L., Xiao J., Huang N., McCouch S. R., Xu Y. et al 1997 Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled haploid, and recombinant inbred populations in rice (Oryza sativa L.). Mol. Gen. Genet. 253, 535–545.PubMedGoogle Scholar
  46. Yuexiong Z., Gang Q. I. N., Qianqian M. A., Minyi W. E. I., Xinghai Y. A. N. G., Zengfeng M. A. et al. 2019 Identification of a major resistance locus Bhp35 to brown planthopper in rice (Oryza sativa L.). Rice Sci. 2.Google Scholar
  47. Zhao Y., Huang J., Wang Z., Jing S., Wang Y., Ouyang Y. et al. 2016 Allelic diversity in an NLR gene BPH9 enables rice to combat planthopper variation. Proc. Natl. Acad. Sci. USA 113, 12850–12855.PubMedGoogle Scholar

Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  • S. Akanksha
    • 1
  • V. Jhansi Lakshmi
    • 1
  • Arun Kumar Singh
    • 1
  • Yamini Deepthi
    • 1
  • P. M. Chirutkar
    • 1
  • Ramdeen
    • 1
  • Divya Balakrishnan
    • 1
  • N. Sarla
    • 1
  • S. K. Mangrauthia
    • 1
  • T. Ram
    • 1
    Email author
  1. 1.ICAR-Indian Institute of Rice ResearchHyderabadIndia

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