Development and characterization of 20 polymorphic microsatellite loci in the deep sea squat lobster, Munida isos Ahyong and Poore, 2004 and cross-amplification in two congeneric species
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Munida isos is a deep sea squat lobster species that is widely distributed across the New Zealand and east Australian region, and is often associated with deep sea vulnerable marine ecosystems. To investigate its population genetic structure and patterns of regional connectivity, microsatellite loci were developed for M. isos from two genomic libraries using the Illumina HiSeq 2500 sequencing platform. Twenty-six loci amplified consistently in M. isos from the Tasman Sea, among which 20 were polymorphic and selectively neutral. Evidence of null alleles was observed at eight loci. Most loci exhibited moderate to high levels of polymorphism, with an average polymorphic information content value of 0.482. The mean number of alleles per locus was 7.45, with a mean expected heterozygosity of 0.520. Thirteen loci exhibited significant deviation from Hardy–Weinberg equilibrium, while only one locus pair was in linkage disequilibrium after false discovery rate correction for multiple testing (\(P < 0.05\)). Cross-species amplification tests revealed that the transferability of 14 loci (70%) was positive for the two congeners M. endeavourae and M. gracilis. The accessibility to new polymorphic microsatellite loci will facilitate population genetic studies and aid in developing conservation and management strategies for vulnerable marine ecosystems.
Keywordsgenetic diversity genetic connectivity management conservation south Pacific Ocean vulnerable marine ecosystems Munididae Munida isos
Squat lobster specimens were supplied for genetic work by the National Institute of Water and Atmospheric (NIWA) Invertebrate Collection, Wellington, New Zealand and Museum Victoria, Melbourne, Australia. Particular special thanks to Ms Sadie Mills and Ms Diana Macpherson of the NIWA Invertebrate Collection and Dr Anna McCallum of the Museum Victoria, for their diligent assistance with loans. Sample collections were supported by funding from the former New Zealand Foundation for Research, Science and Technology, former New Zealand Ministry of Fisheries, Land Information New Zealand, Department of Conservation (New Zealand), GNS Science (New Zealand), Auckland University and Woods Hole Oceanographic Institute (USA). Dr Ashley Rowden of NIWA is thanked for his comments on the manuscript. This work was supported by funding from Victoria University of Wellington to J.P.A.G (SB80802).
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