Advertisement

Journal of Genetics

, 98:36 | Cite as

Identification of sex using SBNO1 gene in the Chinese softshell turtle, Pelodiscus sinensis (Trionychidae)

  • Lan Zhao
  • Xin Wang
  • Qiu-Hong Wan
  • Sheng-Guo FangEmail author
RESEARCH NOTE
  • 21 Downloads

Abstract

The Chinese softshell turtle exhibits ZZ/ZW sex determination. To identify the sex of embryos, juvenile and adult individuals, we designed two pairs of polymerase chain reaction primers, SB1-196, which amplifies a fragment of 196 bp in the female and the other, CK1-482, which amplifies the 482-bp fragment in both the sexes. It is validated in 24 adult turtles of known sex, sampled from three different locations. This one-step sexing technique is rapid and easy to perform and is reported for the first time.

Keywords

polymerase chain reaction sex identification sex chromosome molecular sexing reptile Chinese softshell turtle 

Notes

Acknowledgements

This work was supported by the National Key Programme (2016YFC0503200) from the Ministry of Science and Technology of China, a special grant from the State Forestry Administration, and the Fundamental Research Funds for the Central Universities of China. We are grateful to Xiaoshan Tianfu Bio-Technology, Hangzhou, Zhejiang Province, China, for providing samples of the Chinese softshell turtles of Japanese population.

References

  1. Amstrup S. C., Garner G. W., Cronin M. A. and Patton J. C. 1993 Sex identification of polar bears from blood and tissue samples. Can. J. Zool. 71, 2174–2177.CrossRefGoogle Scholar
  2. Griffiths R., Daan S. and Dijkstra C. 1996 Sex identification in birds using two CHD genes. Proc. Biol. Sci. 263, 1251–1256.CrossRefGoogle Scholar
  3. Han Y. M., Yoo. O. J. and Lee K. K. 1993 Sex determination in single mouse blastomeres by polymerase chain reaction. J. Assist. Reprod. Genet. 10, 151–156.CrossRefGoogle Scholar
  4. Kahn W. N., John J. S. and Quinn T. W. 1998 Chromosome-specific intron size differences in the Avian CHD gene provide an efficient method for sex identification in birds. The Auk. 115, 1074–1078.CrossRefGoogle Scholar
  5. Kawagoshi T., Uno Y., Matsubara K., Matsuda Y. and Nishida C 2009 The ZW micro-sex chromosomes of the Chinese soft-shelled turtle (Pelodiscus sinensis, Trionychidae, Testudines) have the same origin as chicken chromosome 15. Cytogenet. Genome Res. 125, 125–131.CrossRefGoogle Scholar
  6. Kawai A., Nishidaumehara C., Ishijima J., Tsuda Y., Ota H. and Matsuda Y. 2007 Different origins of bird and reptile sex chromosomes inferred from comparative mapping of chicken z-linked genes. Cytogenet. Genome Res. 117, 92–102.CrossRefGoogle Scholar
  7. Kunieda T., Xian M., Kobayashi E., Imamichi T., Morwaki K. and Toyoda Y. 1992 Sexing of mouse preimplantation embryos by detection of Y chromosome-specific sequences using polymerase chain reaction. Biol. Reprod. 46, 692.CrossRefGoogle Scholar
  8. Liu Z. Z., Cai W. Q. and Li S. F. 2004 Analysis of genetic variations of five populations in Trionyx sinensis by RAPD. J. Fish. China 28, 119–126.Google Scholar
  9. Prichard P. C. H. 1979 Encyclopedia of turtles. T. F. H. Publication, New Jersey.Google Scholar
  10. Sacchi P., Soglia D., Maione S., Meneguz G., Campora M. and Rasero R. 2004 A non-invasive test for sex identification in short-toed eagle (Circaetus gallicus). Mol. Cell. Probes 18, 93–196.CrossRefGoogle Scholar
  11. Tang W., Hong W., Wang C., Qian G. and Zhang M. 2015 Karyotype and chromosome G-band analysis of Chinese soft-shelled turtle, Pelodiscus sinensis. J. Shanghai Ocean Univ. 24, 174–181.Google Scholar
  12. Tokita M. and Kuratani S. 2001 Normal embryonic stages of the Chinese softshelled turtle Pelodiscus sinensis (Trionychidae). Zool. Sci. 18, 705–715.CrossRefGoogle Scholar
  13. Wang Z. H., Yin S. J., Gao J., Huang X. and Qian G. 2010 Optimization of RAPD technique of fingerprinting analysis of Pelodiscus sinensis populations. J. Fish. Sci. China. 30, 93–96.Google Scholar
  14. Wang Z., Pascual-Anaya J., Zadissa A. and Li Wengi 2013 The draft genomes of soft-shell turtle and green sea turtle yield insights into the development and evolution of the turtle-specific body plan. Nat. Genet. 46, 701–706.CrossRefGoogle Scholar
  15. Xiao Y. M., Chen L. L., Chen H. G. and R. R. Zhao 2005 RAPD analysis of genomic DNA in the Pelodiscus sinensis. J. Nat. Sci. Hunan Normal Univ. 28, 72–75Google Scholar
  16. Zhang D., Xiong M., Bu H., Wang D., Li s., Yao M. et al. 2016 Sex identification of the masked palm civet (Paguma larvata), using noninvasive hair samples. Conserv. Genet. Res. 8, 207–209.Google Scholar
  17. Zhao E. and Adler K. 1993 Herpetology of China. University of Science and Technology of Chengdu Press, Chengdu.Google Scholar

Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  1. 1.The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and State Conservation Centre for Gene Resources of Endangered Wildlife, College of Life SciencesZhejiang UniversityHangzhouPeople’s Republic of China

Personalised recommendations