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Journal of Genetics

, 98:2 | Cite as

Identification of microsatellite markers and their application in yellow catfish (Pseudobagrus fulvidraco Richardson, 1846) population genetics of Korea

  • Jeong-Nam YuEmail author
  • Sang-Ki Kim
  • Jin Sagong
  • Shi Hyun Ryu
  • Byungsoo Chae
Research Note
  • 13 Downloads

Abstract

Microsatellite markers from a fresh water yellow catfish, Pseudobagrus fulvidraco, were developed by whole-genome sequencing in the Ion S5 system. Of the 40 chosen sets of microsatellite markers, with tetra-repeat and penta-repeat motifs, from a total 19,743 sequence, only 13 markers were successfully applied in 78 individual fish sampled to detect genomic variability from four natural populations of Korea. On an average, the number of alleles per marker was 6.7. The observed heterozygosity varied from 0.048 to 0.810. Twelve microsatellite markers conformed to Hardy–Weinberg equilibrium and none exhibited significant linkage disequilibrium. In yellow catfish, genetic differentiation among four natural populations was further supported by \(F_{\mathrm{ST}}\) (\(P<0.05\)) and STRUCTURE analysis. The microsatellite markers identified could facilitate genetic diversity and population structure studies and thus aid in conservation of the yellow catfish.

Keywords

yellow catfish population genetics conservation genetics Korea microsatellites Pseudobagrus fulvidraco 

Notes

Acknowledgements

This project was supported by the grant ‘Research for Conservation and Management of Freshwater Biodiversity (2017)’ funded by the Nakdonggang National Institute of Biological Resources (NNIBR).

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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  1. 1.Nakdonggang National Institute of Biological ResourcesSangju-siSouth Korea
  2. 2.Institute of Freshwater EcologyDaeguSouth Korea

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