Cytosine deletion at AP2-box region of HSP70 promoter and its influence on semen quality traits in crossbred bulls
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Present investigation identified the effect of genetic polymorphism within the AP2 box region 12 located at bovine HSP70 promoter on certain semen quality traits among crossbred bulls revealed the fact that superior capability of wild type promoter genotype over the deletion mutation at HSP70 promoter may partly elucidate better semen quality traits for their contribution in selection of better quality bulls.
Although artificial insemination (AI) is now extensively used in cattle breeding, there is about 40–50% loss in bull semen quality during the freezing–thawing process, including sperm motility, effective survival time and antioxidant enzymes activity (Hu et al. 2011). Recently, studies have been carried out in our laboratory to unveil the genetic basis of semen quality traits in cattle (Kumar et al.2014, 2015a, 2015b). It is well known fact that mammalian spermatogenetic cells are sensitive to heat stress (Yin et al. 1997). Studies have established that heat shock...
KeywordsAP2 box promoter HSP70 semen quality crossbred bulls
We are thankful to Director, ICAR-CIRC for providing necessary facilities to conduct the work. We also acknowledge Science and Engineering Research Board, Government of India, for providing financial support under the project YSS/2014/000279 to RD. We are also thankful to the Military Farm, Meerut, India, for providing the animals used in the present experiment.
- Box G. E. and Cox D. R. 1964 An analysis of transformations . J. Roy Stat. Soc. 26, 211–252.Google Scholar
- Hammersdtedt R. H., Graham J. K. and Nolan J. P. 1990 Cryopreservation of mammalian sperm: what we ask them to survive. J. Androl. 11, 73–88.Google Scholar
- Kamaruddin M., Kroetsch T., Basrur P. K. and King W. A. 1996 Heat shock protein 70 in bovine semen. Biol. Reprod. 54, 222.Google Scholar
- Kumar S., Deb R., Singh U., Ganguly I., Mandal D. K., Singh R. et al. 2014 SNPs at exonic region of Aquaporin-7 (AQP-7) gene may effects semen quality parameters among crossbred bulls . J. Genet. 93, Online Resources. e108–e112.Google Scholar
- Kumar S., Deb R., Singh U., Ganguly I., Mandal D. K., Singh R. et al. 2015a Bovine circadian locomotor output cyclic kaput (CLOCK) and cluster in (CLU) mRNA quantitation in ejaculated crossbred bull spermatozoa. Reprod. Domest. Anim., doi: 10.1111/rda.12522.
- Kumar S., Singh U., Deb R., Tyagi S., Mandal D. K., Kumar M. et al. 2015b A SNP (g.358A >T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility. Meta Gene, (doi: 10.1016/j.mgene.2015.07.004).
- Kumar S., Singh U., Ganguly I., Deb R., Singh R., Mann S. et al. 2015c Protamine 3 expressions in crossbred bull spermatozoa may not be a prognostic marker for differentiating good and poor quality semen. African J. Biotechnol. 13, 20.Google Scholar
- Kuo Y. H., Huang S. Y. and Lee Y. P. 1997 Effects of breed and season on semen characteristics of boars in subtropical area . J. Chin. Soc. Vet. Sci. 23, 114–122.Google Scholar
- Nascimento J. M., Shi L. Z., Tam J., Chandsawangbhuwana C., Durrant B., Botvinick E. L. et al. 2008 Comparison of glycolysis andoxidative phosphorylation as energy sources for mammalian spermmotility, using the combination of fluorescence imaging, laser tweezers, and real-time automated tracking and trapping . J. Cell Physiol. 217, 745–751.CrossRefPubMedPubMedCentralGoogle Scholar
- Nover L. 1991 Heat shock response. CRC Press, Boca Raton, USA.Google Scholar
- Schwerin M., Sanftleben H. and Grupe S. 2003 Genetic predisposition for productive life is associated with functional inactivation of a AP2-binding site in the promoter of the stress protein 70.1-encoding gene in cattle. Arch. Tierz., Dummerstorf. 46, 177–185.Google Scholar
- Shrum K., Lester T., Rorie R., Reiter S., Looper M. and Rosenkrans J. C. F. 2010 Effects of heat shock protein 70 haplotype and tall fescue variety on bull sperm characteristics. Arkansas Agric. Res. Stat. Res. Ser. 584, 39–44.Google Scholar