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Journal of Genetics

, Volume 95, Issue 2, pp 341–347 | Cite as

Identification of genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig

  • YUUTA KOMATSU
  • SHIN SUKEGAWA
  • MAI YAMASHITA
  • NAOKI KATSUDA
  • BIN TONG
  • TAKESHI OHTA
  • HIROYUKI KOSE
  • TAKAHISA YAMADAEmail author
RESEARCH ARTICLE

Abstract

Suppression subtractive hybridization was used to identify genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig. Two subtracted cDNA populations were generated from musculus longissimus muscle tissues of selected pigs with extreme expected breeding values at the age of 100 kg. Three upregulated genes (EEF1A2, TSG101 and TTN) and six downregulated genes (ATP5B, ATP5C1, COQ3, HADHA, MYH1 and MYH7) in pig with genetic propensity for higher growth rate were identified by sequence analysis of 12 differentially expressed clones selected by differential screening following the generation of the subtracted cDNA population. Real-time PCR analysis confirmed difference in expression profiles of the identified genes in musculus longissimus muscle tissues between the two Landrace weanling pig groups with divergent genetic propensity for growth rate. Further, differential expression of the identified genes except for the TTN was validated by Western blot analysis. Additionally, the eight genes other than the ATP5C1 co-localized with the same chromosomal positions as QTLs that have been previously identified for growth rate traits. Finally, the changes of expression predicted from gene function suggested association of upregulation of expression of the EEF1A2, TSG101 and TTN genes and downregulation of the ATP5B, ATP5C1, COQ3, HADHA, MYH1 and MYH7 gene expression with increased growth rate. The identified genes will provide an important insight in understanding of the molecular mechanism underlying growth rate in Landrace pig breed.

Keywords

expression profile growth rate Landrace pig skeletal muscle suppression subtractive hybridization 

Notes

Acknowledgement

This work was supported by a grant-in-aid for Scientific Research (C) (no. 24580406) from Ministry of Education, Science, Sports, and Culture of Japan.

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Copyright information

© Indian Academy of Sciences 2016

Authors and Affiliations

  • YUUTA KOMATSU
    • 1
  • SHIN SUKEGAWA
    • 2
  • MAI YAMASHITA
    • 1
  • NAOKI KATSUDA
    • 1
  • BIN TONG
    • 1
  • TAKESHI OHTA
    • 1
  • HIROYUKI KOSE
    • 3
  • TAKAHISA YAMADA
    • 1
    Email author
  1. 1.Faculty of Agriculture, Department of AgrobiologyNiigata UniversityNiigataJapan
  2. 2.Research and Development Center, NH Foods Ltd.TsukubaJapan
  3. 3.Department of Natural SciencesInternational Christian UniversityMitakaJapan

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