Abundance and characteristics of microsatellite markers in Gansu zokor (Eospalax cansus), a fossorial rodent endemic to the Loess plateau, China
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Microsatellites, also known as simple sequence repeats (SSRs) or short tandem repeats (STRs) are regions widely distributed in the genome that contain tandem repeats of short nucleotide sequences. Microsatellite motifs are conserved in species. Their abundance, codominance, robustness and ease of amplification by polymerase chain reaction (PCR) make them good genetic markers for a wide range of studies (Geleta and Grausgruber 2012). They have long been utilized for population level studies in the fields of genetics, ecology, evolution conservation and management (Selkoe and Toonen 2006). Unlike nonspecific markers, such as amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD), microsatellites are single-locus DNA markers that require specific primers for PCR amplifications (Zane et al. 2002) and, therefore, designing primers is an important step in their development.
Early, development of...
KeywordsZokor subterranean rodents microsatellites genomic sequencing
Funding for this project was provided by the Ministry of Agriculture Public Benefit Research Foundation of China (grant no. 201203041), and the Science and Research Project of the Education Department of Gansu Province, China (grant no. 1102-02). We thank Duckchul Park, Diana Prada and Julia Allwood for laboratory assistance.
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