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Journal of Genetics

, Volume 92, Issue 2, pp 205–212 | Cite as

Preliminary genetic linkage maps of Chinese herb Dendrobium nobile and D. moniliforme

  • SHANGGUO FENG
  • HONGYAN ZHAO
  • JIANGJIE LU
  • JUNJUN LIU
  • BO SHENEmail author
  • HUIZHONG WANGEmail author
Research Article

Abstract

Dendrobium is an endangered genus in the orchid family with medicinal and horticultural value. Two preliminary genetic linkage maps were constructed using 90 F1 progeny individuals derived from an interspecific cross between D. nobile and D. moniliforme (both, 2n = 38), using random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR). A total of 286 RAPD loci and 68 ISSR loci were identified and used for genetic linkage analysis. Maps were constructed by double pseudo-testcross mapping strategy using the software Mapmaker/EXP ver. 3.0, and Kosambi map distances were constructed using a LOD score ≥ 4 and a recombination threshold of 0.4. The resulting frame map of D. nobile was 1474 cM in total length with 116 loci distributed in 15 linkage groups; and the D. moniliforme linkage map had 117 loci placed in 16 linkage groups spanning 1326.5 cM. Both maps showed 76.91% and 73.59% genome coverage for D. nobile and D. moniliforme, respectively. These primary maps provide an important basis for genetic studies and further medicinal and horticultural traits mapping and marker-assisted selection in Dendrobium breeding programmes.

Keywords

Dendrobium double pseudo-testcross genetic linkage RAPD ISSR 

Notes

Acknowledgements

This research was funded in part by the National Natural Science Foundation (no. 30670199, 30870180), the Zhejiang Scientific and Technological Program (2008C12081), the Hangzhou Scientific and Technological Program (20080432T06), and Qianjiang Scholar Program.

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Copyright information

© Indian Academy of Sciences 2013

Authors and Affiliations

  1. 1.Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal PlantsHangzhou Normal UniversityHangzhouPeople’s Republic of China

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