Isolation, characterization and mapping of genes differentially expressed during fibre development between Gossypium hirsutum and G. barbadense by cDNA-SRAP
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Gossypium hirsutum and G. barbadense are two cultivated tetraploid cotton species with differences in fibre quality. The fibre of G. barbadense is longer, stronger and finer than that of G. hirsutum. To isolate genes expressed differently between the two species during fibre development, cDNA-SRAP (sequence-related amplified polymorphism) was applied. This technique was used to analyse genes at different stages of fibre development in G. hirsutum cv. Emian22 and G. barbadense acc. 3-79, the parents of our interspecific mapping population. A total of 4096 SRAP primer combinations were used to screen polymorphism between the DNA of the parents, and 275 highly polymorphic primers were picked out to analyse DNA and RNA from leaves and fibres at different developmental stages of the parents. A total of 168 DNA fragments were isolated from gels and sequenced: 54, 30, 38 and 41 from fibres of 5, 10, 15 and 20 days post-anthesis, respectively, and five from multi stages. To genetically map these sequences, 104 sequence-specific primers were developed and were used to screened polymorphism between the mapping parents. Finally, six markers were mapped on six chromosomes of our backbone interspecific genetic map. This work can give us a primary knowledge of differences in mechanism of fibre development between G. hirsutum and G. barbadense.
Keywordscotton fibre development cDNA-SRAP genetic mapping
This work was financially supported by the National 863 High Technology Project (no. 2012AA101108-3) and the National Basic Research Program (no. 2010CB126001).
- Hinchliffe D. J., Meredith W. R., Yeater K. M., Kim H. J., Woodward A. W., Chen Z. J. and Triplett B. A. 2010 Near-isogenic cotton germplasm lines that differ in fibre-bundle strength have temporal differences in fibre gene expression patterns as revealed by comparative high-throughput profiling. Theor. Appl. Genet. 120, 1347–1366.PubMedCrossRefGoogle Scholar
- John M. E. 1995 Characterization of a cotton (Gossypium hirsutum L.) fibre-mRNA (Fb-b6). Plant Physiol. 107, 1478–1486.Google Scholar
- Kosambi D. D. 1994 The estimation of map distance from recombination values. Ann. Eugen. 12, 172–175.Google Scholar
- Lacape J. M., Nguyen T. B., Courtois B., Belot J. L., Giband M., Gourlot J. P. et al. 2005 QTL analysis of cotton fibre quality using multiple Gossypium hirsutum × Gossypium barbadense backcross generations. Crop Sci. 45, 123–140.Google Scholar
- Lang A. G. 1938 The origin of lint and fuzz hairs of cotton. J. Agric. Res. 56, 507–521.Google Scholar
- Wu Y., Llewellyn D. J., White R., Ruggiero K., Al-Ghazi Y. and Dennis E. S. 2007 Laser capture microdissection and cDNA microarrays used to generate gene expression profiles of the rapidly expanding fibre initial cells on the surface of cotton ovules. Planta 226, 1475–1490.PubMedCrossRefGoogle Scholar
- Zhu L. F., Tu L. L., Zhen F. C., Liu D. Q. and Zhang X. L. 2005 An improved simple protocol for isolation of high quality RNA from Gossypium spp. suitable for cDNA library construction. Acta Agromomica Sin. 31, 1657–1659.Google Scholar