Journal of Genetics

, Volume 90, Issue 1, pp 111–117

Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons

  • MARIELA NIEVES
  • EDIVALDO H. C. DE OLIVEIRA
  • PAULO J. S. AMARAL
  • CLEUSA Y. NAGAMACHI
  • JULIO C. PIECZARKA
  • MARÍA C. MÜHLMANN
  • MARTA D. MUDRY
Research Article

Abstract

The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity and composition of heterochromatin in this genus. In this context, we use fluorescence in situ hybridization (FISH) to analyse this genomic region and discuss its possible role in the diversification of Cebus. We used a heterochromatin probe for chromosome 11 of Cebus libidinosus (11qHe+ CLI probe), obtained by chromosome microdissection. Twenty-six specimens belonging to the families Atelidae, Cebidae, Callitrichidae and Pithecidae (Platyrrhini) were studied. Fourteen out of 26 specimens were Cebus (Cebidae) individuals of C. libidinosus, C. xanthosternos, C. apella, C. nigritus, C. albifrons, C. kaapori and C. olivaceus. In Cebus specimens, we found 6 to 22 positive signals located in interstitial and telomeric positions along the different species. No hybridization signal was observed among the remaining Ceboidea species, thus reinforcing the idea of a Cebus-specific heterochromatin composed of a complex system of repetitive sequences.

Keywords

primate evolution chromosome microdissection FISH heterochromatin Platyrrhini Cebus 

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Copyright information

© Indian Academy of Sciences 2011

Authors and Affiliations

  • MARIELA NIEVES
    • 1
    • 3
    • 4
  • EDIVALDO H. C. DE OLIVEIRA
    • 2
  • PAULO J. S. AMARAL
    • 2
  • CLEUSA Y. NAGAMACHI
    • 2
  • JULIO C. PIECZARKA
    • 2
  • MARÍA C. MÜHLMANN
    • 3
    • 4
  • MARTA D. MUDRY
    • 1
    • 4
  1. 1.Grupo de Investigación en Biología Evolutiva (GIBE), Laboratorio 46, 4° piso, Facultad de Ciencias Exactas y Naturales (FCEyN)Universidad de Buenos Aires (UBA)Ciudad Autónoma de Buenos AiresArgentina
  2. 2.Laboratório de Citogenética, Departamento de Genética, Centro de Ciências Biológicas, Rua Augusto CorrêaBelémBrazil
  3. 3.Laboratorio de Citogenética Molecular, Instituto Nacional de Tecnología IndustrialUniversidad de San MartínSan MartínArgentina
  4. 4.Consejo Nacional de Investigaciones Cientificas y TécnicasCiudad Autónoma de Buenos AiresArgentina

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