Journal of Genetics

, Volume 90, Issue 1, pp 59–66

Mapping of shoot fly tolerance loci in sorghum using SSR markers

  • J. V. PATIL
  • P. L. KULWAL
Research Article


Sorghum (Sorghum bicolor (L.) Moench) is one of the most important crops in the semiarid regions of the world. One of the important biotic constraints to sorghum production in India is the shoot fly which attacks sorghum at the seedling stage. Identification of the genomic regions containing quantitative trait loci (QTLs) for resistance to shoot fly and the linked markers can facilitate sorghum improvement programmes through marker-assisted selection. A simple sequence repeat (SSR) marker- based skeleton linkage map of two linkage groups of sorghum was constructed in a population of 135 recombinant inbred lines (RIL) derived from a cross between IS18551 (resistant to shoot fly) and 296B (susceptible to shoot fly). A total of 14 SSR markers, seven each on linkage groups A and C were mapped. Using data of different shoot fly resistance component traits, one QTL which is common for glossiness, oviposition and dead hearts was detected following composite interval mapping (CIM) on linkage group A. The phenotypic variation explained by this QTL ranged from 3.8%–6.3%. Besides the QTL detected by CIM, two more QTLs were detected following multi-trait composite interval mapping (MCIM), one each on linkage groups A and C for the combinations of traits which were correlated with each other. Results of the present study are novel as we could find out the QTLs governing more than one trait (pleiotropic QTLs). The identification of pleiotropic QTLs will help in improvement of more than one trait at a time with the help of the same linked markers. For all the QTLs, the resistant parent IS18551 contributed resistant alleles.


sorghum shoot fly SSR linkage mapping QTL mapping pleiotropic QTL 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Supplementary material

12041_2011_46_MOESM1_ESM.pdf (511 kb)
(PDF 510 kb)


  1. Aruna C. and Padmaja P. G. 2009 Evaluation of genetic potential of shoot fly resistant sources in sorghum Sorghum bicolor (L.) Moench. J. Agric. Sci. 147, 71–80.CrossRefGoogle Scholar
  2. Bantilan M. C. S., Deb U. K., Gowda C. L. L., Reddy B. V. S., Obilana A. B. and Evenson R. E. (ed.) 2004 Sorghum genetic enhancement: research process, dissemination and impacts, pp. 320. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, India.Google Scholar
  3. Bhattramakki D., Dong J., Chhabra A. K. and Hart G. E. 2000 An integrated SSR and RFLP linkage map of Sorghum bicolor (L.) Moench. Genome 43, 988–1002.PubMedGoogle Scholar
  4. Chen Y. and Lubberstedt T. 2010 Molecular basis of trait correlations. Trends Plant Sci. 15, 454–461.PubMedCrossRefGoogle Scholar
  5. Deeming J. C. 1971 Some species of Atherigona rondani (Diptera: Muscidae) from northern Nigeria, with special reference to those injurious to cereal crops. Bull. Entomol. Res. 61, 133–190.CrossRefGoogle Scholar
  6. Folkertsma R. T., Sajjanar G. M., Reddy B. V. S., Sharma H. C. and Hash C. T. 2003 Genetic mapping of QTL associated with sorghum shoot fly (Atherigona soccata) resistance in sorghum (Sorghum bicolor). In Final abstracts guide, plant and animal genome XI, p. 462. Town and Country Hotel, San Diego, USA (
  7. Gupta P. K., Kumar J., Mir R. R. and Kumar A. 2010 Marker-assisted selection as a component of conventional plant breeding. Plant Breed. Rev. 33, 145–217.CrossRefGoogle Scholar
  8. Hallali M. S., Gowda B. T. S., Kulkarni K. A. and Goud J. V. 1982 Inheritance of resistance to shootfly (Atherigona soccata Rond.) in sorghum. SABRAO J. 14, 165–170.Google Scholar
  9. Haussmann B. I. G., Hess D. E., Seetharama N., Welz H. G. and Geiger H. H. 2002 Construction of a combined sorghum linkage map from two recombinant inbred populations using AFLP, SSR, RFLP, and RAPD markers, and comparison with other sorghum maps. Theor. Appl. Genet. 105, 629–637.PubMedCrossRefGoogle Scholar
  10. Kamatar M. Y. and Salimath P. M. 2003 Morphological traits of sorghum associated with resistance to shoot fly, Atherigona soccata Rondani. Ind. J. Plant Prot. 31, 73–77.Google Scholar
  11. Karanjkar R. R., Chundurwar R. D. and Borikar S. T. 1992 Correlation and path analysis of shoot fly resistance in sorghum. J. Maharashtra Agric. Univ. 17, 389–391.Google Scholar
  12. Kosambi D. D. 1944 The estimation of map distances from recombination values. Ann. Eugen. 12, 172–175.CrossRefGoogle Scholar
  13. Kumar A. A., Reddy B. V. S., Sharma H. C. and Ramaiah B. 2008 Shoot fly (Atherigona soccata) resistance in improved grain sorghum hybrids. E-Journal of SAT Agric. Res. 6, 1–4. doi:10.3914/lCRISAT.0039.Google Scholar
  14. Lander E. S., Green P., Abrahamson J., Barlow A. and Daly M. J. 1987 MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1, 174–181.PubMedCrossRefGoogle Scholar
  15. Michelmore W. R., Paran I. and Kesseli R. V. 1991 Identification of marker linked to diseases resistance genes by bulked segregant analysis, a rapid method to detect the markers in specific genetic region by using the segregating population. Proc. Natl. Acad. Sci. USA 88, 9828–9832.PubMedCrossRefGoogle Scholar
  16. Nwaze K. F. 1997 Screening for resistance to sorghum shoot fly. In Plant resistance to insect in sorghum. (ed. H. C. Sharma, F. Singh and K. F. Nwaze), pp. 35–37. International Crops Research Institute for the Semi- Arid Tropics, Patancheru, India.Google Scholar
  17. Nwaze K. F., Reddy Y. V. R. and Soman P. 1990 The role of leaf surface wetness in larval behaviour of the sorghum shoot fly Atherigona soccata. Entomol. Exp. Appl. 56, 187–195.CrossRefGoogle Scholar
  18. Patel G. M. and Sukhani T. R. 1990 Biophysical plant characters associated with shoot fly resistance. Indian J. Ent. 52, 14–17.Google Scholar
  19. Sajjanar G. M. 2002 Genetic analysis and molecular mapping of components of resistance to shoot fly (Atherigona soccata) in sorghum (Sorghum bicolor (L.) Moench). Ph.D. thesis, University of Agricultural Sciences, Dharwad, India.Google Scholar
  20. Satish K., Srinivas G., Madhusudhana R., Padmaja P. G., Reddy R. N., Mohan S. M. and Seetharama N. 2009 Identification of quantitative trait loci for resistance to shoot fly in sorghum (Sorghum bicolor (L.) Moench). Theor. Appl. Genet. 119, 1425–1439.PubMedCrossRefGoogle Scholar
  21. Sharma H. C. 1997 Plant resistance to insects: Basic principles. In Plant resistance to insects in sorghum (ed. H. C. Sharma, F. Singh and K. F. Nwanze) pp. 24–31. ICRISAT, Patancheru, India.Google Scholar
  22. Venkateswarlu D. 2009 Marker-trait association study for shoot fly tolerance in sorghum using SSR markers. M.Sc. thesis, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, India.Google Scholar
  23. Wang S., Basten C. J. and Zeng Z. B. 2007 Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, USA (

Copyright information

© Indian Academy of Sciences 2011

Authors and Affiliations

    • 1
    • 1
    • 2
    • 3
  • J. V. PATIL
    • 4
  • P. L. KULWAL
    • 1
    • 4
  1. 1.Department of Agricultural BotanyDr. Panjabrao Deshmukh Agricultural UniversityAkolaIndia
  2. 2.Sorghum Research UnitDr. Panjabrao Deshmukh Agricultural UniversityAkolaIndia
  3. 3.Pulses Research UnitDr. Panjabrao Deshmukh Agricultural UniversityAkolaIndia
  4. 4.State Level Biotechnology CentreMahatma Phule Agricultural UniversityRahuriIndia

Personalised recommendations