Journal of Biosciences

, 44:127 | Cite as

Sp7/osterix positively regulates dlx2b and bglap to affect tooth development and bone mineralization in zebrafish larvae

  • Zhongjian Chen
  • Zhiyun Song
  • Jinjing Yang
  • Jian Huang
  • Hongbing JiangEmail author


Osterix (or Sp7) is an important transcription factor that promotes osteoblast differentiation by modulating the expression of a range of target genes. Although many studies have focused on Osterix/Sp7 regulatory mechanisms, the detailed functions have not been fully elucidated. Toward this end, in this study, we used CRISPR/Cas9 technology to knock out the zebrafish sp7 gene, and then analyzed its phenotype and biological function. Two knockout sp7 mutant lines were successfully obtained. The bone mineralization level was significantly reduced in the zebrafish sp7−/− homozygote, resulting in abnormal tooth development in the larvae. Quantitative real-time polymerase chain reaction showed that loss of sp7 led to down-regulated expression of the dlx2b and bglap genes related to tooth development and bone mineralization, respectively. Moreover, cell transfection experiments demonstrated that Sp7 directly regulates the expression of dlx2b and bglap through Sp7-binding sites on the promoter regions of these two genes. Overall, this study provides new insight into the role of Sp7 in bone mineralization and tooth development.


bglap bone mineralization dlx2b Osterix/sp7 tooth development 



This work was supported by Grants from Suzhou people’s Welfare and Science Items (SYS201659).


  1. Arnold WH, Naumova KI, Naumova EA and Gaengler P 2008 Comparative qualitative and quantitative assessment of biomineralization of tooth development in man and zebrafish (Danio rerio). Anat. Rec. 291 571–576CrossRefGoogle Scholar
  2. Baek WY, Lee MA, Jung JW, Kim SY, Akiyama H, de Crombrugghe B and Kim JE 2009 Positive regulation of adult bone formation by osteoblast-specific transcription factor osterix. J. Bone Miner. Res. 24 1055–1065CrossRefGoogle Scholar
  3. Gibert Y, Samarut E, Pasco-Viel E, Bernard L, Borday-Birraux V, Sadier A, Labbe C, Viriot L and Laudet V 2015 Altered retinoic acid signalling underpins dentition evolution. Proc. Biol. Sci. 282 Scholar
  4. Hosoya A, Yukita A, Ninomiya T, Hiraga T, Yoshiba K, Yoshiba N, Kasahara E and Nakamura H 2013 Localization of SUMOylation factors and Osterix in odontoblast lineage cells during dentin formation and regeneration. Histochem. Cell Biol. 140 201–211CrossRefGoogle Scholar
  5. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G and Zhang F 2013 DNA targeting specificity of RNA-guided Cas9 nucleases. Nat. Biotechnol. 31 827–832CrossRefGoogle Scholar
  6. Jackman WR, Draper BW and Stock DW 2004 Fgf signaling is required for zebrafish tooth development. Dev. Biol. 274 139–157CrossRefGoogle Scholar
  7. Kague E, Witten PE, Soenens M, Campos CL, Lubiana T, Fisher S, Hammond C, Brown KR, Passos-Bueno MR and Huysseune A 2018 Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth. Dev. Biol. 435 176–184CrossRefGoogle Scholar
  8. Kawai S, Yamauchi M and Amano A 2017 Zinc-finger transcription factor Odd-skipped related 1 regulates cranial bone formation. J. Bone Miner. Metab. 36 640–647CrossRefGoogle Scholar
  9. Koga T, Matsui Y, Asagiri M, Kodama T, de Crombrugghe B, Nakashima K and Takayanagi H 2005 NFAT and Osterix cooperatively regulate bone formation. Nat. Med. 11 880–885CrossRefGoogle Scholar
  10. Lee MH, Kwon TG, Park HS, Wozney JM and Ryoo HM 2003 BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2. Biochem. Biophys. Res. Commun. 309 689–694CrossRefGoogle Scholar
  11. Merlo GR, Zerega B, Paleari L, Trombino S, Mantero S and Levi G 2000 Multiple functions of Dlx genes. Int. J. Dev. Biol. 44 619–626PubMedGoogle Scholar
  12. Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR and de Crombrugghe B 2002 The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell 108 17–29CrossRefGoogle Scholar
  13. Nishimura R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, Kiyonari H, Shioi G, Yamaguchi A, Tsumaki N, Akiyama H and Yoneda T 2012 Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification. J. Biol. Chem. 287 33179–33190CrossRefGoogle Scholar
  14. Ortuno MJ, Susperregui AR, Artigas N, Rosa JL and Ventura F 2010 Osterix induces Col1a1 gene expression through binding to Sp1 sites in the bone enhancer and proximal promoter regions. Bone 52 2013 548–556Google Scholar
  15. Perez-Campo FM, Santurtun A, Garcia-Ibarbia C, Pascual MA, Valero C, Garces C, Sanudo C, Zarrabeitia MT and Riancho JA 2016 Osterix and RUNX2 are transcriptional regulators of sclerostin in human bone. Calcif .Tissue Int. 99 302–309CrossRefGoogle Scholar
  16. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y and Zhang F 2013 Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell 154 1380–1389CrossRefGoogle Scholar
  17. Reinholt FP, Hultenby K, Oldberg A and Heinegard D 1990 Osteopontin–a possible anchor of osteoclasts to bone. Proc. Natl. Acad. Sci. USA 87 4473–4475CrossRefGoogle Scholar
  18. Renn J and Winkler C 2014 Osterix/Sp7 regulates biomineralization of otoliths and bone in medaka (Oryzias latipes). Matrix Biol. 34 193–204CrossRefGoogle Scholar
  19. Slavkin HC, Hu CC, Sakakura Y, Diekwisch T, Chai Y, Mayo M, Bringas P Jr, Simmer J, Mak G, Sasano Y, et al. 1992 Gene expression, signal transduction and tissue-specific biomineralization during mammalian tooth development. Crit. Rev. Eukaryot. Gene Expr. 2 315–329PubMedGoogle Scholar
  20. Tang W, Li Y, Osimiri L and Zhang C 2011 Osteoblast-specific transcription factor Osterix (Osx) is an upstream regulator of Satb2 during bone formation. J. Biol. Chem. 286 32995–33002CrossRefGoogle Scholar
  21. Termine JD, Kleinman HK, Whitson SW, Conn KM, McGarvey ML and Martin GR 1981 Osteonectin, a bone-specific protein linking mineral to collagen. Cell 26 99–105CrossRefGoogle Scholar
  22. Thomas BL, Porteus MH, Rubenstein JL and Sharpe PT 1995 The spatial localization of Dlx-2 during tooth development. Connect. Tissue Res. 32 27–34CrossRefGoogle Scholar
  23. Verreijdt L, Debiais-Thibaud M, Borday-Birraux V, Van der Heyden C, Sire JY and Huysseune A 2006 Expression of the dlx gene family during formation of the cranial bones in the zebrafish (Danio rerio): differential involvement in the visceral skeleton and braincase. Dev. Dyn. 235 1371–1389CrossRefGoogle Scholar
  24. Walker MB and Kimmel CB 2007 A two-color acid-free cartilage and bone stain for zebrafish larvae. Biotech. Histochem. 82 23–28CrossRefGoogle Scholar
  25. Weinreb M, Shinar D and Rodan GA 1990 Different pattern of alkaline phosphatase, osteopontin, and osteocalcin expression in developing rat bone visualized by in situ hybridization. J. Bone Miner. Res. 5 831–842CrossRefGoogle Scholar
  26. Wise SB and Stock DW 2010 bmp2b and bmp4 are dispensable for zebrafish tooth development. Dev. Dyn. 239 2534–2546CrossRefGoogle Scholar
  27. Yu T, Graf M, Renn J, Schartl M, Larionova D, Huysseune A, Witten PE and Winkler C 2017 A vertebrate-specific and essential role for osterix in osteogenesis revealed by gene knockout in the teleost medaka. Development 144 265–271CrossRefGoogle Scholar
  28. Yuan Q, Zhao M, Tandon B, Maili L, Liu X, Zhang A, Baugh EH, Tran T, Silva RM, Hecht JT, Swindell EC, Wagner DS and Letra A 2017 Role of WNT10A in failure of tooth development in humans and zebrafish. Mol. Genet. Genomic Med. 5 730–741CrossRefGoogle Scholar
  29. Yun-Feng W, Matsuo N, Sumiyoshi H and Yoshioka H 2010 Sp7/Osterix up-regulates the mouse pro-alpha3(V) collagen gene (Col5a3) during the osteoblast differentiation. Biochem. Biophys. Res. Commun. 394 503–508CrossRefGoogle Scholar
  30. Zhang C, Cho K, Huang Y, Lyons JP, Zhou X, Sinha K, McCrea PD and de Crombrugghe B 2008 Inhibition of Wnt signaling by the osteoblast-specific transcription factor Osterix. Proc. Natl. Acad. Sci. USA 105 6936–6941CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  • Zhongjian Chen
    • 1
    • 2
    • 3
  • Zhiyun Song
    • 3
  • Jinjing Yang
    • 4
  • Jian Huang
    • 4
  • Hongbing Jiang
    • 1
    • 2
    Email author
  1. 1.Jiangsu Key Laboratory of Oral DiseasesNanjing Medical UniversityNanjingChina
  2. 2.Department of Oral and Maxillofacial SurgeryThe Affiliated Hospital of Stomatology, Nanjing Medical UniversityNanjingChina
  3. 3.The Affiliated Stemmatological Hospital of Soochow UniversitySuzhouChina
  4. 4.School of Biology and Basic Medical SciencesSoochow UniversitySuzhouChina

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