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Journal of Biosciences

, 44:101 | Cite as

Down-regulation effects of IFN-α on p11, 5-htr1b and 5-HTR4 protein levels were affected by NH4CL or MG132 treatment in SH-sy5y cells

  • Jiqiang Guo
  • Huaxia Ding
  • Zhuangwei Lv
  • Junna Jiao
  • Hui Wang
  • Yanhong JiEmail author
Article
  • 23 Downloads

Abstract

In previous studies, we found interferon-α (IFN-α) could reduce protein levels of p11, 5-hydroxytryptamine receptor 1b (5-HT1b) and 5-hydroxytryptamine receptor 4 (5-HT4), but does not influence their messenger RNA levels in SH-sy5y cells. Thus, we investigated the post-transcriptional modulation of these molecules by IFN-α. SH-sy5y cells were treated with IFN-α, NH4Cl or MG132 alone or in combination, and then the protein levels of p11, 5-HT1b and 5-HT4 were analyzed by western blots. The regulatory effects of p11 on 5-HT1b and 5-HT4 were also determined in p11 knock-down cells. NH4Cl but not MG132 could reverse the protein level of p11 in IFN-α-treated SH-sy5y cells. MG132 could recover the protein levels of 5-HT1b and 5-HT4 in p11 knock-down cells. The down-regulation effects of IFN-α on p11, 5-HT1b and 5-HT4 were associated with the lysosome and ubiquitin–proteasome-mediated pathways. p11 was identified as a potent regulator to modulate the ubiquitination of 5-HT1b and 5-HT4. Therefore, it could be potential target therapies in IFN-α-induced depression.

Keywords

5-Hydroxytryptamine receptor 4 5-hydroxytryptamine receptor 1b interferon-α MG132 NH4Cl p11 

Notes

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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  • Jiqiang Guo
    • 1
    • 2
  • Huaxia Ding
    • 3
  • Zhuangwei Lv
    • 2
  • Junna Jiao
    • 2
  • Hui Wang
    • 2
  • Yanhong Ji
    • 1
    Email author
  1. 1.Department of Pathogenic Biology and Immunology, School of Basic Medical SciencesXi’an Jiaotong University Health Science CentreXi’anPeople’s Republic of China
  2. 2.Department of Immunology, School of Basic Medical SciencesXinxiang Medical UniversityXinxiangPeople’s Republic of China
  3. 3.Third Affiliated HospitalXinxiang Medical UniversityXinxiangPeople’s Republic of China

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