14-3-3 proteins mediate the localization of Centrin2 to centrosome
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14-3-3ε and 14-3-3γ localize to the centrosome and regulate centrosome duplication, by inhibiting cdc25C function. As 14-3-3γ and 14-3-3ε form a complex with centrosomal proteins, we asked if this ability was required to regulate centrosome duplication. The results in this report demonstrate that 14-3-3ε and 14-3-3γ form a complex with Centrin2 and that the binding site is located in the N-terminal EF hand in Centrin2, EF1. A Centrin2 mutant that does not form a complex with 14-3-3 proteins displays a punctate cytoplasmic localization and does not localize to the centrosome. These results suggest that in addition to negatively regulating centrosome duplication as previously reported, 14-3-3 proteins might also be required for centriole biogenesis by regulating the localization of Centrin2 at the centrosome.
KeywordsCentrosome centriole Centrin2 14-3-3
We thank Dr Erich Nigg, for his kind gift of the EGFP C2 Centrin2 construct and the ACTREC microscopy facility for help with the imaging experiments in this report. The work was funded by grants from the Department of Biotechnology, Govt. of India (BT/PR4181/BRB/10/1147/2012 and BT/PR8351/MED/30/995/2013) to SND.
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