Restriction enzymes and their use in molecular biology: An overview
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Restriction enzymes have been identified in the early 1950s of the past century and have quickly become key players in the molecular biology of DNA. Forty years ago, the scientists whose pioneering work had explored the activity and sequence specificity of these enzymes, contributing to the definition of their enormous potential as tools for DNA characterization, mapping and manipulation, were awarded the Nobel Prize. In this short review, we celebrate the history of these enzymes in the light of their many different uses, as these proteins have accompanied the history of DNA for over 50 years representing active witnesses of major steps in the field.
KeywordsCloning DNA manipulation history restriction enzymes
- Danna K and Nathans D 1971 Specific cleavage of Simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae. Proc. Natl. Acad. Sci. USA 5 75–81Google Scholar
- Khan S, Ullah MW, Siddique R, Nabi G, Manan S, Yousaf M and Hou H 2016 Role of recombinant DNA technology to improve life. Int. J. Genomics 2016 1–14Google Scholar
- Miller JC, Tan S, Qiao G, Barlow KA, Wang J, Xia DF, Meng X, Paschon DE, Leung E, Hinkley SJ, Dulay GP, Hua KL, Ankoudinova I, Cost GJ, Urnov FD, Zhang HS, Holmes MC, Zhang L, Gregory PD and Rebar EJ 2011 A TALE nuclease architecture for efficient genome editing. Nat. Biotechnol. 29 143–148CrossRefGoogle Scholar