Comparison of Treponema pallidum genomes for the prediction of resistance genes
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Syphilis is a sexually transmitted infection caused by Treponema pallidum, which is highly prevalent in several countries, including Brazil. The use of bioinformatics’ tools for the identification of resistance genes is an important practice for the study of microorganisms, such as T. pallidum. In this study, the complete genomes of 43 strains of T. pallidum, isolated from different countries, were analyzed. A total of 41,514 sequences were obtained, and compared against prokaryote resistance gene databases using BLASTn, BLASTx and RGI for gene alignment and prediction. From the alignments, it was possible to identify antibiotic resistance genes for each strain. The genes identified in each comparison were grouped according to the antibiotic category in which they show resistance to. The antibiotic-resistant genes related to drugs used to treat syphilis were grouped separately. The in silico tools used have shown to be effective in identifying resistance genes in genomes of T. pallidum strains. Due to the lack of research and accurate information regarding the antibiotic resistance genes in T. pallidum, this study serves as a basis for studies in molecular biology whose aim is the identification of these genes, besides being a reference to help in the control and treatment of this infection.
KeywordsBioinformatics genomes molecular biology resistance genes syphilis
This work was partially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq grant 440245/2018-4), Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT grants 092/2015 and 041/2017), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, grant 001) and Universidade Federal da Grande Dourados (UFGD). KES received a scholarship from CAPES.
- Aids MdSSdVeSPNdDe 2005 Diretrizes para o controle da sífilis congênita: Ministério da Saúde BrasíliaGoogle Scholar
- Grimes M, Sahi SK, Godornes BC, Tantalo LC, Roberts N, Bostick D, Marra CM and Lukehart SA 2012 Two mutations associated with macrolide resistance in Treponema pallidum: Increasing prevalence and correlation with molecular strain type in Seattle, Washington. Sex. Transm. Dis. 39 954–958CrossRefGoogle Scholar
- Newman L, Rowley J, Vander Hoorn S, Wijesooriya NS, Unemo M, Low N, Stevens G, Gottlieb S, Kiarie J and Temmerman M 2015 Global estimates of the prevalence and incidence of four curable sexually transmitted infections in 2012 based on systematic review and global reporting. PLoS One 10 e0143304CrossRefGoogle Scholar
- Workgroup AGP 2012 Prevalence of the 23S rRNA A2058G point mutation and molecular subtypes in Treponema pallidum in the United States, 2007 to 2009. Sex. Transm. Dis. 39 794–798Google Scholar