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Construction of a dietary-cure Saccharomyces cerevisiae expressing long-acting glucagon-like peptide-1 and investigation of its hypoglycemic activity in type 2 diabetes mellitus mouse model

  • Pingzhe Jiang
  • Chao Ma
  • Junfeng Hao
  • Yike Han
  • Minggang LiEmail author
Article
  • 2 Downloads

Abstract

Bio-drug is a new type of beneficial biology expressing therapeutic peptides (protein) as orally administrated medicine to treat diseases, in particular, chronic diseases like diabetes. In order to develop recombinant yeast strains as a bio-drug which could effectively ameliorate type 2 diabetes, an integrating expression vector pNK1-PGK that could successfully express green fluorescent protein (GFP) in Saccharomyces cerevisiae was constructed to demonstrate the normality of the function. A pNK1-PGK vector, which expresses 10 tandem repeats of long-acting glucagon-like peptide-1(10laGLP-1), was cloned and then transformed into the S. cerevisiae INVSc1. The long-acting GLP-1 hypoglycemic yeast (LHY168) that grew rapidly and expressed 10laGLP-1 stably was screened by uracil-deficient plates and Western blot. The expression quantity of 10laGLP-1 reached 1.56 mg/g cell wet weight. Moreover, the oral administration of LHY168 significantly declined the blood glucose in type 2 diabetic mice that were constructed through co-induction of streptozotocin (STZ) and high-fat and high-sugar diet.

Keywords

Long-acting GLP-1 hypoglycemic yeast (LHY) long-acting glucagon-like peptide-1(10laGLP-1) recombinant yeast type 2 diabetes 

Notes

Acknowledgements

This work is supported by the National Natural Science Foundation of China (31771066) and the Key Technologies R&D Program of Tianjin (18YFZCSY00030 and 18YFZCNC01200).

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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  • Pingzhe Jiang
    • 1
    • 2
  • Chao Ma
    • 2
  • Junfeng Hao
    • 2
  • Yike Han
    • 2
  • Minggang Li
    • 2
    Email author
  1. 1.Liaoning Ocean and Fisheries Science Research InstituteDalianChina
  2. 2.Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life ScienceNankai UniversityTianjinChina

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