GFP fluorescence: A few lesser-known nuggets that make it work
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Use of Green Fluorescent Protein (GFP) as a marker has revolutionized biological research in the last few decades. In this brief commentary, we reflect upon the success story of GFP and highlight a few lesser-known facets about GFP that add up to its usefulness.
KeywordsGFP REES FRAP lateral diffusion
enhanced green fluorescent protein
enhanced yellow fluorescent protein
fluorescence recovery after photobleaching
green fluorescent protein
G protein-coupled receptor
red edge excitation shift
AC gratefully acknowledges support from SERB Distinguished Fellowship (Department of Science and Technology, India). PS thanks the Council of Scientific and Industrial Research for the award of a Shyama Prasad Mukherjee Fellowship. AC is a Distinguished Visiting Professor at Indian Institute of Technology (Bombay), and Adjunct Professor at Tata Institute of Fundamental Research (Mumbai), RMIT University (Melbourne, Australia) and Indian Institute of Science Education and Research (Kolkata). Some of the work described in this article was carried out by former members of AC’s research group whose contributions are gratefully acknowledged. We thank members of the Chattopadhyay laboratory for their comments and discussions.
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