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Journal of Biosciences

, Volume 40, Issue 1, pp 125–135 | Cite as

BDP-30, a systemic resistance inducer from Boerhaavia diffusa L., suppresses TMV infection, and displays homology with ribosome-inactivating proteins

  • Shalini Srivastava
  • HN Verma
  • Aparana Srivastava
  • Vivek Prasad
Article

Abstract

Root extract of Boerhaavia diffusa L. induced systemic resistance in tobacco against Tobacco mosaic virus. A 30 kDa protein was isolated as the active component, called BDP-30 on the basis of the molecular weight and source plant. BDP-30, a glycoprotein, was found to be temperature and protease resistant. It was basic, possessing a pI greater than 9.0. In-gel proteolytic digestion of BDP-30 generated two peptides that possessed the amino acid sequence KLYDIPPLR and KVTLPYSGNYER by LC/MS/MS. Both peptides shared absolute sequence identity with trichosanthin, a ribosome-inactivating protein from Trichosanthes kirilowii, and a 78% and 100% homology respectively with an RIP from Bryonia dioica, bryodin. Further, effort was made to look at the fate of TMV in induced resistant Nicotiana tabacum cv. Xanthi, a systemic host of the virus, at specified days after inoculation in control and treated plants. TMV coat protein (CP) was detected by immunoblot 7 days post inoculation up to 21 days in the control set, but not in treated resistant plants. TMV RNA was detected by RT-PCR using TMV-CP specific primers. Resistant tobacco did not show presence of TMV RNA up to 21 days of inoculation. This suggests that BDP-30 may be suppressing TMV replication.

Keywords

Basic antiviral glycoprotein BDP-30 Boerhaavia diffusa induced systemic resistance ribosome-inactivating proteins 

Notes

Acknowledgements

SS acknowledges the award of the UGC/CSIR NET JRF no. 276/JRF(UGC)CSIR-1989.

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Copyright information

© Indian Academy of Sciences 2015

Authors and Affiliations

  • Shalini Srivastava
    • 1
  • HN Verma
    • 1
    • 2
  • Aparana Srivastava
    • 1
  • Vivek Prasad
    • 1
  1. 1.Molecular Plant Virology Lab, Department of BotanyLucknow UniversityLucknowIndia
  2. 2.Jaipur National UniversityJaipurIndia

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