Journal of Biosciences

, Volume 38, Issue 2, pp 229–237 | Cite as

Candidate gene makers for Candidatus Liberibacter asiaticus for detecting citrus greening disease

  • Madhugiri Nageswara-RaoEmail author
  • Mike Irey
  • Stephen M Garnsey
  • Siddarame GowdaEmail author
Brief communication


Citrus Huanglongbing (HLB) also known as citrus greening is one of the most devastating diseases of citrus worldwide. The disease is caused by Candidatus Liberibacter bacterium, vectored by the psyllid Diaphorina citri Kuwayama and Trioza erytreae Del Guercio. Citrus plants infected by the HLB bacterium may not show visible symptoms sometimes for years following infection. The aim of this study was to develop effective gene-specific primer pairs for polymerase chain reaction based method for quick screening of HLB disease. Thirty-two different gene-specific primer pairs, across the Ca. Liberibacter asiaticus genome, were successfully developed. The possibility of these primer pairs for cross-genome amplification across ‘Ca. Liberibacter africanus’ and ‘Ca. Liberibacter americanus’ were tested. The applicability of these primer pairs for detection and differentiation of Ca Liberibacter spp. is discussed.


Citrus greening Candidatus Liberibacter cross-amplification DNA markers polymerase chain reaction 



This research was supported by the Florida Citrus Production Research Advisory Council, Florida, USA. The authors also thank Dr. William O Dawson for permission to use his laboratory and greenhouse facilities.


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Copyright information

© Indian Academy of Sciences 2013

Authors and Affiliations

  1. 1.University of Florida, IFAS, Citrus Research and Education CenterLake AlfredUSA
  2. 2.Department of Plant SciencesThe University of TennesseeKnoxvilleUSA
  3. 3.US Sugar CorporationClewistonUSA

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