Journal of Biosciences

, Volume 37, Issue 1, pp 91–101 | Cite as

Leptin regulates proliferation and apoptosis of colorectal carcinoma through PI3K/Akt/mTOR signalling pathway

  • Di Wang
  • Jian Chen
  • Hui Chen
  • Zhi Duan
  • Qimei Xu
  • Meiyan Wei
  • Lianghua Wang
  • Meizuo Zhong
Article

Abstract

Epidemiological studies have indicated that obesity is associated with colorectal cancer. The obesity hormone leptin is considered as a key mediator for cancer development and progression. The present study aims to investigate regulatory effects of leptin on colorectal carcinoma. The expression of leptin and its receptor Ob-R was examined by immunohistochemistry in 108 Chinese patients with colorectal carcinoma. The results showed that leptin/Ob-R expression was significantly associated with T stage, TNM stage, lymph node metastasis, distant metastasis, differentiation and expression of p-mTOR, p-70S6 kinase, and p-Akt. Furthermore, the effects of leptin on proliferation and apoptosis of HCT-116 colon carcinoma cells were determined. The results showed that leptin could stimulate the proliferation and inhibit the apoptosis of HCT-116 colon cells through the PI3K/Akt/mTOR pathway. Ly294002 (a PI3K inhibitor) and rapamycin (an mTOR inhibitor) could prevent the regulatory effects of leptin on the proliferation and apoptosis of HCT-116 cells via abrogating leptin-mediated PI3K/Akt/mTOR pathway. All these results indicated that leptin could regulate proliferation and apoptosis of colorectal carcinoma through the PI3K/Akt/mTOR signalling pathway.

Keywords

Colorectal carcinoma HCT-116 leptin mammalian target of rapamycin (mTOR) phosphatidylinositol 3-kinase PI3K 

Notes

Acknowledgements

The authors would like to thank Jiajia Wang for excellent technical assistance and Fengjie Guo for figure preparation. We also thank Jia-Jia Wang for critical and constructive reading of the manuscript. This work was supported by the Grant from Science and Technology Agency of Hunan Province, No.2010SK3179

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Copyright information

© Indian Academy of Sciences 2011

Authors and Affiliations

  • Di Wang
    • 1
    • 2
  • Jian Chen
    • 3
  • Hui Chen
    • 2
  • Zhi Duan
    • 2
  • Qimei Xu
    • 2
  • Meiyan Wei
    • 2
  • Lianghua Wang
    • 2
  • Meizuo Zhong
    • 1
  1. 1.Departments of Cancer, Xiangya HospitalCentral South UniversityChangshaPR, China
  2. 2.Department of PathologyThe First Hospital of ChangshaChangshaPR, China
  3. 3.Department of General SurgeryThe First Hospital of ChangshaChangshaPeople’s Republic of China

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