Journal of Biosciences

, Volume 36, Issue 1, pp 139–151 | Cite as

Overexpression of the mitogen-activated protein kinase gene OsMAPK33 enhances sensitivity to salt stress in rice (Oryza sativa L.)

  • Seong-Kon Lee
  • Beom-Gi Kim
  • Taek-Ryoun Kwon
  • Mi-Jeong Jeong
  • Sang-Ryeol Park
  • Jung-Won Lee
  • Myung-Ok Byun
  • Hawk-Bin Kwon
  • Benjamin F Matthews
  • Choo-Bong Hong
  • Soo-Chul Park
Article

Abstract

Mitogen-activated protein kinases (MAPK) signalling cascades are activated by extracellular stimuli such as environmental stresses and pathogens in higher eukaryotic plants. To know more about MAPK signalling in plants, a MAPK cDNA clone, OsMAPK33, was isolated from rice. The gene is mainly induced by drought stress. In phylogenetic analysis, OsMAPK33 (Os02g0148100) showed approximately 47–93% identity at the amino acid level with other plant MAPKs. It was found to exhibit organ-specific expression with relatively higher expression in leaves as compared with roots or stems, and to exist as a single copy in the rice genome. To investigate the biological functions of OsMAPK33 in rice MAPK signalling, transgenic rice plants that either overexpressed or suppressed OsMAPK33 were made. Under dehydration conditions, the suppressed lines showed lower osmotic potential compared with that of wild-type plants, suggesting a role of OsMAPK33 in osmotic homeostasis. Nonetheless, the suppressed lines did not display any significant difference in drought tolerance compared with their wild-type plants. With increased salinity, there was still no difference in salt tolerance between OsMAPK33-suppressed lines and their wild-type plants. However, the overexpressing lines showed greater reduction in biomass accumulation and higher sodium uptake into cells, resulting in a lower K+/Na+ ratio inside the cell than that in the wild-type plants and OsMAPK33-suppressed lines. These results suggest that OsMAPK33 could play a negative role in salt tolerance through unfavourable ion homeostasis. Gene expression profiling of OsMAPK33 transgenic lines through rice DNA chip analysis showed that OsMAPK33 altered expression of genes involved in ion transport. Further characterization of downstream components will elucidate various biological functions of this novel rice MAPK.

Keywords

Abiotic stress tolerance MAPK microarray rice transgenic plants 

Abbreviations

ABA

abscisic acid

IPTG

isopropyl-β-d-thiogalactose

MAPK

mitogen-activated protein kinases

Notes

Acknowledgements

This work was supported by a research grant from the National Academy of Agricultural Science, Rural Development Administration, Republic of Korea.

Supplementary material

12038_2011_9002_MOESM1_ESM.pdf (167 kb)
ESM 1 (PDF 166 kb)

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Copyright information

© Indian Academy of Sciences 2011

Authors and Affiliations

  • Seong-Kon Lee
    • 4
    • 1
  • Beom-Gi Kim
    • 1
  • Taek-Ryoun Kwon
    • 1
  • Mi-Jeong Jeong
    • 1
  • Sang-Ryeol Park
    • 1
  • Jung-Won Lee
    • 1
  • Myung-Ok Byun
    • 1
  • Hawk-Bin Kwon
    • 2
  • Benjamin F Matthews
    • 3
  • Choo-Bong Hong
    • 4
  • Soo-Chul Park
    • 1
  1. 1.Department of Agricultural BiotechnologyNational Academy of Agricultural Science, Rural Development AdministrationSuwonRepublic of Korea
  2. 2.Department of Biomedical ScienceSun Moon UniversityAsanRepublic of Korea
  3. 3.Soybean Genomics and Improvement Lab, USDA-ARSBeltsvilleUSA
  4. 4.Department of Biological SciencesSeoul National UniversitySeoulRepublic of Korea

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