Journal of Biosciences

, Volume 34, Issue 6, pp 891–898 | Cite as

Pharmacological effects of two cytolysins isolated from the sea anemone Stichodactyla helianthus

  • T. García
  • D. Martinez
  • A. Palmero
  • C. Soto
  • M. Tejuca
  • F. Pazos
  • R. Menéndez
  • C. Alvarez
  • A. Garateix
Article

Abstract

Sticholysins I and II (St I/II) are cytolysins purified from the sea anemone Stichodactyla helianthus. In this study, we show their pharmacological action on guinea-pig and snail models in native and pH-denatured conditions in order to correlate the pharmacological findings with the pore-forming activity of both isoforms. In guinea-pig erythrocytes (N = 3), St II possessed higher haemolytic activity in comparison with St I and this activity was lost at an alkaline pH. In molluscan central neurons (N = 30), they irreversibly decreased the amplitude of the cholinergic response; St I (EC 50 0.6 µmolL−1) was more potent than St II (EC50 >6.6 µmolL−1) and they both increased the duration of the action potential; these effects were absent at an alkaline pH. In guinea-pig isolated atrium (N = 25), both increased the amplitude of the contraction force, but St II was more potent than St I (EC50 0.03 µmolL−1 and 0.3 µmolL−1, respectively) and this effect persisted at an alkaline pH. In summary, both cytolysins have neuroactive and cardioactive properties. The main mechanism in molluscan neurons seems to be associated with the cytolytic activity of these molecules, whereas in guinea-pig atrium, the existence of an additional pharmacological mechanism might be contributing to the observed effect.

Keywords

Anemone cardioactive cytolysins neuroactive pharmacology 

Abbreviations used

AA

arachidonic acid

Ach

acetylcholine

AP

action potential

EqTx

equinatoxin

GABA

gamma aminobutyric acid

HMg

heteractis magnificalysin

PBS

phosphate buffered saline

RBC

red blood cell

RMP

resting membrane potential

St I/II

sticholysins I and II

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References

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Copyright information

© Indian Academy of Sciences 2009

Authors and Affiliations

  • T. García
    • 1
  • D. Martinez
    • 2
  • A. Palmero
    • 1
  • C. Soto
    • 2
  • M. Tejuca
    • 2
  • F. Pazos
    • 2
  • R. Menéndez
    • 1
  • C. Alvarez
    • 2
  • A. Garateix
    • 1
  1. 1.Centro de Bioproductos MarinosLa HabanaCuba
  2. 2.Centro de Estudios de Proteínas, Facultad de BiologíaUniversidad de La HabanaLa HabanaCuba

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