Journal of Biosciences

, Volume 33, Issue 3, pp 345–354 | Cite as

Differential dynamics of splicing factor SC35 during the cell cycle

  • Kaushlendra Tripathi
  • Veena K. ParnaikEmail author


Pre-mRNA splicing factors are enriched in nuclear domains termed interchromatin granule clusters or nuclear speckles. During mitosis, nuclear speckles are disassembled by metaphase and reassembled in telophase in structures termed mitotic interchromatin granules (MIGs). We analysed the dynamics of the splicing factor SC35 in interphase and mitotic cells. In HeLa cells expressing green fluorescent protein (GFP)-SC35, this was localized in speckles during interphase and dispersed in metaphase. In telophase, GFP-SC35 was highly enriched within telophase nuclei and also detected in MIGs. Fluorescence recovery after photobleaching (FRAP) experiments revealed that the mobility of GFP-SC35 was distinct in different mitotic compartments. Interestingly, the mobility of GFP-SC35 was 3-fold higher in the cytoplasm of metaphase cells compared with interphase speckles, the nucleoplasm or MIGs. Treatment of cells with inhibitors of cyclin-dependent kinases (cdks) caused changes in the organization of nuclear compartments such as nuclear speckles and nucleoli, with corresponding changes in the mobility of GFP-SC35 and GFP-fibrillarin. Our results suggest that the dynamics of SC35 are significantly influenced by the organization of the compartment in which it is localized during the cell cycle.


Cell cycle dynamics FRAP analysis mitotic interchromatin granules splicing factor SC35 

Abbreviations used


alternate splicing factor


cyclin-dependent kinase


Cdc2-like kinase




foetal bovine serum


fluorescein isothiocyanate


fluorescence recovery after photobleaching


green fluorescent protein


glutathione S-transferase


interchromatin granule cluster




monoclonal antibody


mitotic interchromatin granule


NOR-associated patch


nucleolar organizing region


phosphate-buffered saline


polyvinylidene fluoride


relative fluorescence intensity




sodium dodecyl sulphate-polyacrylamide gel electrophoresis


small nuclear ribonucleoprotein


small nucleolar RNA


serine/arginine-rich protein kinase 1


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Copyright information

© Indian Academy of Sciences 2008

Authors and Affiliations

  1. 1.Centre for Cellular and Molecular BiologyHyderabadIndia

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