Journal of Biosciences

, Volume 32, Issue 4, pp 755–761 | Cite as

Hyaluronidase and collagenase inhibitory activities of the herbal formulation Triphala guggulu

  • Venil N. Sumantran
  • Asavari A. Kulkarni
  • Abhay Harsulkar
  • Asmita Wele
  • Soumya J. Koppikar
  • Rucha Chandwaskar
  • Vishakha Gaire
  • Madhuri Dalvi
  • Ulhas V. Wagh
Article

Abstract

Myrrh (guggulu) oleoresin from the Commiphora mukul tree is an important component of antiarthritic drugs in Ayurvedic medicine. Clinical data suggest that elevated levels of hyaluronidase and collagenase type 2 enzymes contribute significantly to cartilage degradation. Triphala guggulu (TG) is a guggulu-based formulation used for the treatment of arthritis. We assessed the chondroprotective potential of TG by examining its effects on the activities of pure hyaluronidase and collagenase type 2 enzymes. Triphala shodith guggulu (TSG), an intermediate in the production of TG, was also examined. A spectrophotometric method was used to assay Hyaluronidase activity, and to detect potential Hyaluronidase inhibitors. Aqueous and hydro-alcoholic extracts of TSG showed weak but dose-dependent inhibition of hyaluronidase activity. In contrast, the TG formulation was 50 times more potent than the TSG extract with respect to hyaluronidase inhibitory activity. A validated X-ray film-based assay was used to measure the gelatinase activity of pure collagenase type 2. Hydro-alcoholic extracts of the TG formulation were 4 times more potent than TSG with respect to collagenase inhibitory activity. Components of Triphala were also evaluated for their inhibitory activities on hyaluronidase and collagenase. This is the first report to show that the T2 component of Triphala (T. chebula) is a highly potent hyaluronidase and collagenase inhibitor. Thus, the TG formulation inhibits two major enzymes that can degrade cartilage matrix. Our study provides the first in vitro preclinical evidence of the chondroprotective properties of TG.

Keywords

Collagenase hyaluronidase Triphala guggulu (TG) Triphala shodith guggulu (TSG) 

Abbreviations used

ADAMTS

disintegrin containing metalloproteinases with thrombospondin motifs

HA

hyaluronic acid

HPTLC

high-performance thin layer chromatography

MMP

matrix metalloproteinase

OA

osteoarthritis

TG

Triphala guggulu

TSG

Triphala shodith guggulu

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Copyright information

© Indian Academy of Sciences 2007

Authors and Affiliations

  • Venil N. Sumantran
    • 1
  • Asavari A. Kulkarni
    • 1
  • Abhay Harsulkar
    • 1
  • Asmita Wele
    • 2
  • Soumya J. Koppikar
    • 1
  • Rucha Chandwaskar
    • 1
  • Vishakha Gaire
    • 2
  • Madhuri Dalvi
    • 2
  • Ulhas V. Wagh
    • 1
  1. 1.Interactive Research School for Health Affairs (IRSHA)Bhartiya Vidyapeeth Deemed University Medical College CampusDhankawadi, PuneIndia
  2. 2.Department of Ayurvedic StudiesBhartiya Vidyapeeth Deemed University Medical College CampusDhankawadi, PuneIndia

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