Hyaluronidase and collagenase inhibitory activities of the herbal formulation Triphala guggulu
- 300 Downloads
Myrrh (guggulu) oleoresin from the Commiphora mukul tree is an important component of antiarthritic drugs in Ayurvedic medicine. Clinical data suggest that elevated levels of hyaluronidase and collagenase type 2 enzymes contribute significantly to cartilage degradation. Triphala guggulu (TG) is a guggulu-based formulation used for the treatment of arthritis. We assessed the chondroprotective potential of TG by examining its effects on the activities of pure hyaluronidase and collagenase type 2 enzymes. Triphala shodith guggulu (TSG), an intermediate in the production of TG, was also examined. A spectrophotometric method was used to assay Hyaluronidase activity, and to detect potential Hyaluronidase inhibitors. Aqueous and hydro-alcoholic extracts of TSG showed weak but dose-dependent inhibition of hyaluronidase activity. In contrast, the TG formulation was 50 times more potent than the TSG extract with respect to hyaluronidase inhibitory activity. A validated X-ray film-based assay was used to measure the gelatinase activity of pure collagenase type 2. Hydro-alcoholic extracts of the TG formulation were 4 times more potent than TSG with respect to collagenase inhibitory activity. Components of Triphala were also evaluated for their inhibitory activities on hyaluronidase and collagenase. This is the first report to show that the T2 component of Triphala (T. chebula) is a highly potent hyaluronidase and collagenase inhibitor. Thus, the TG formulation inhibits two major enzymes that can degrade cartilage matrix. Our study provides the first in vitro preclinical evidence of the chondroprotective properties of TG.
KeywordsCollagenase hyaluronidase Triphala guggulu (TG) Triphala shodith guggulu (TSG)
disintegrin containing metalloproteinases with thrombospondin motifs
high-performance thin layer chromatography
Triphala shodith guggulu
Unable to display preview. Download preview PDF.
- Banerjee P 2002 Quality control of herbal drugs (New Delhi: Business Horizons) pp 723–725Google Scholar
- Chunekar K C and Pandeya G S 1995 Bhavaprakashnighantu 10th edition (Varanasi: Chaukhabha Bharati Academy) p. 206Google Scholar
- Harsulkar A M, Giri A P, Gupta V S, Sainani M N, Deshpande V V, Patankar A G and Ranjekar P K 1998 Characterization of Helicoverpa armigera gut proteinases and their interaction with proteinase inhibitors using gel X-ray film contact print technique; Electrophoresis 19 1397–1402PubMedCrossRefGoogle Scholar
- Indian herbal Pharmacopeia Revised edn. 2002 (Mumbai: Indian Drug Manufacturers Association) pp 493–496Google Scholar
- Pelletier J P and Martel-Pelletier J 1993 Effects of nimesulide and naproxen on the degradation and metalloprotease synthesis of human osteoarthritic cartilage; Drugs (Suppl. 1) 46 134–139Google Scholar
- Shaha N C 1985 The encyclopedia of ayurvedic formulations (New Delhi: B Jain Publishers) Vol 2, pp 360–361Google Scholar
- Svoboda R E 1992 Ayurvedic life health and longevity 1st edition (New Delhi: Penguin Books) p 221, 258–25Google Scholar