Molecular Neurobiology

, Volume 55, Issue 5, pp 4127–4135 | Cite as

TRPV4 Activation Contributes Functional Recovery from Ischemic Stroke via Angiogenesis and Neurogenesis

  • Chun-Kai Chen
  • Po-Yuan Hsu
  • Tzu-Ming Wang
  • Zhi-Feng Miao
  • Ruey-Tay Lin
  • Suh-Hang H. JuoEmail author


The endothelial transient receptor potential cation channel subfamily V member 4 (TRPV4) plays a crucial role in vascular remodeling; however, TRPV4-mediated angiogenesis after ischemic neuronal death as a neurorestorative strategy has not yet been thoroughly examined. In this study, we first tested whether TRPV4 activation can improve functional recovery in rats subjected to transient brain ischemia. The possible mechanisms for TRPV4 activation-promoted functional recovery were explored. A TRPV4 agonist, 4α-phorbol 12,13-didecanoate (4α-PDD), was intravenously injected via the tail vein at 6 h and 1, 2, 3, 4 days after ischemic stroke. The treatment reduced infarct volume by almost 50% (14.7 ± 3.7 vs. 29.2 ± 6.2%; p < 0.0001) and improved functional outcomes (p = 0.03) on day 5. To explore the therapeutic mechanism, we measured endothelial nitric oxide synthase (eNOS) expression and phosphorylation, vascular endothelial growth factor A (VEGFA) signaling, and neural stem/progenitor cells (NPCs). TRPV4 activation significantly increased eNOS expression and phosphorylation (serine 1177) by more than 2-fold in the ischemic region. The expressions of VEGFA and VEGF receptor-2 were significantly higher in the treated animals, especially an increase of the proangiogenic VEGFA164a isoform while a decrease of the antiangiogenic VEGFA165b isoform. We evaluated angiogenesis by detecting microvessel density in ischemic region. Using the immunohistochemistry staining, we found that 4α-PDD treatment caused a 3.4-fold increase of microvessel density (p < 0.0001). In addition, NPC proliferation and migration in the ischemic hemisphere were increased by 3-fold and 5-fold, respectively. In conclusion, our data suggest that TRPV4 activation by 4α-PDD may improve poststroke functional improvement through angiogenesis and neurogenesis.


TRPV4 Nitric oxide synthase Angiogenesis Neurogenesis Ischemic stroke 



4α-Phorbol 12,13-didecanoate


Blood–brain barrier


Endothelial progenitor cells


Endothelial cells


Fluid shear stress


Garcia score


Endothelial nitric oxide synthase


Neural stem/progenitor cells


Nitric oxide


Subventricular zone


Transient middle carotid artery occlusion


Transient receptor potential vanilloid 4


Vascular endothelial growth factor A


VEGF receptor-2



We thank JY. Wang (Division of Colorectal Surgery, Department of Surgery, Kaohsiung Medical University) for technical support. This work was supported by the following funding: Ministry of Science and Technology (Taiwan, R.O.C.; MOST 103-2314-B-075-076-MY3, MOST 104-2745-B-037-001, MOST 105-2314-B-039-050, MOST 103-2314-B-037-027-MY2) and National Health Research Institutes (Taiwan, R.O.C.; NHRI-EX106-10605PI).

Compliance with Ethical Standards

All experimental procedures were approved by the Institutional Animal Ethical Committee Kaohsiung Medical University and were conducted according to the Guide for the Care and Use of Laboratory Animal of the National Institute of Health.

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

12035_2017_625_Fig7_ESM.gif (15 kb)
Supplementary Fig. 1

Schematic illustration of the experimental design. Transient middle cerebral artery occlusion (tMCAO) was inducted by 2-h occlusion in right internal carotid artery. 4a-phorbol-12,13-didecanoate (4α-PDD) was used as an agonist for endothelial transient receptor potential vanilloid 4.* represents the test points for neurological deficits score (NDS). ** represents the test points for infarct volume. # represents the test points for Garcia score (GS). (GIF 15 kb)

12035_2017_625_MOESM1_ESM.tif (77 kb)
High resolution image (TIFF 76 kb)
12035_2017_625_Fig8_ESM.gif (35 kb)
Supplementary Fig. 2

Delayed deaths are not observed with 4α-PDD treatment. Mortality was assessed during 5 d after tMCAO in 4α-PDD group (n = 23), and control group (treated with DMSO, n = 26). Mortality at day 5 was not significantly different. (GIF 34 kb)

12035_2017_625_MOESM2_ESM.tif (207 kb)
High resolution image (TIFF 206 kb)
12035_2017_625_MOESM3_ESM.docx (13 kb)
Supplementary Table S1 (DOCX 13 kb)
12035_2017_625_MOESM4_ESM.docx (14 kb)
Supplementary Table S2 (DOCX 14 kb)


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Chun-Kai Chen
    • 1
    • 2
  • Po-Yuan Hsu
    • 3
  • Tzu-Ming Wang
    • 3
  • Zhi-Feng Miao
    • 4
  • Ruey-Tay Lin
    • 5
  • Suh-Hang H. Juo
    • 3
    • 6
    • 7
    • 8
    • 9
    Email author
  1. 1.Department of Physical Medicine and RehabilitationKaohsiung Medical University HospitalKaohsiungTaiwan
  2. 2.Graduate Institute of Medicine, Collage of MedicineKaohsiung Medical UniversityKaohsiungTaiwan
  3. 3.Department of Medical ResearchChina Medical University HospitalTaichungTaiwan
  4. 4.Division of Colorectal Surgery, Department of SurgeryKaohsiung Medical University HospitalKaohsiungTaiwan
  5. 5.Department of NeurologyKaohsiung Medical University HospitalKaohsiungTaiwan
  6. 6.Graduate Institute of Biomedical SciencesChina Medical UniversityTaichungTaiwan
  7. 7.Institute of New Drug DevelopmentChina Medical UniversityTaichungTaiwan
  8. 8.Brain Disease Research CenterChina Medical UniversityTaichungTaiwan
  9. 9.Center for Myopia and Eye DiseaseChina Medical UniversityTaichungTaiwan

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