Molecular Neurobiology

, Volume 54, Issue 4, pp 2901–2921 | Cite as

An Antagomir to MicroRNA-106b-5p Ameliorates Cerebral Ischemia and Reperfusion Injury in Rats Via Inhibiting Apoptosis and Oxidative Stress

  • Pengfei Li
  • Meihong Shen
  • Feng Gao
  • Jinping Wu
  • Jiahui Zhang
  • Fengmeng Teng
  • Chunbing Zhang
Article

Abstract

We previously observed that microRNA miR-106b-5p significantly increased in serum of patients with acute ischemic stroke. The present study was to determine whether miR-106b-5p antagomir can protect against cerebral ischemia/reperfusion (I/R) injury and elucidate its underlying mechanisms. Middle cerebral artery occlusion (MCAO) was operated on male Sprague Dawley rats. MiR-106b-5p antagomir significantly decreased neurological deficit scores, infarct volumes, and neuronal injury. Furthermore, miR-106b-5p antagomir markedly reduced malondialdehyde (MDA) content, restored superoxide dismutase (SOD) activity, increased the expression of myeloid cell leukemia-1 (Mcl-1) and B cell lymphoma-2 (Bcl-2), and decreased the expression of Bax in the ischemic cortex. In PC12 cells, miR-106b-5p inhibitor increased the Mcl-1 and Bcl-2 expression, which provided protection against glutamate-induced apoptosis and oxidative damage, as evidenced by decreased lactate dehydrogenase (LDH) release, and enhanced SOD activity. Notably, luciferase reported assay proved Mcl-1 was the target gene of miR-106b-5p. In conclusion, our data indicates that the neuroprotective effects of miR-106b-5p antagomir on cerebral I/R injury are associated with its inhibition of apoptosis and oxidative stress, suggesting a potential therapeutic target for ischemic stroke.

Keywords

MiR-106b-5p Ischemic stroke Middle cerebral artery occlusion Apoptosis Oxidative stress 

Abbreviations

MiRNA

MicroRNA

MCAO

Middle cerebral artery occlusion

CNS

Central nerve system

IS

Ischemic stroke

I/R

Ischemia/reperfusion

MDA

Malondialdehyde

Bcl-2

B cell lymphoma-2

LDH

Lactate dehydrogenase

SOD

Superoxide dismutase

ROS

Reactive oxygen species

TTC

2,3,5-Triphenyltetrazolium chloride

HE

Hematoxylin and eosin

MRI

Magnetic resonance imaging

FITC

Fluorescein isothiocyanate

ROS

Reactive oxygen species

3′-UTR

3′-Untranslated region

MCM7

Mini chromosome maintenance protein 7

SEM

Standard error of the mean

PI

Propidium iodide

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Pengfei Li
    • 1
  • Meihong Shen
    • 2
  • Feng Gao
    • 1
  • Jinping Wu
    • 3
  • Jiahui Zhang
    • 3
  • Fengmeng Teng
    • 1
  • Chunbing Zhang
    • 1
    • 3
  1. 1.Department of Clinical Laboratory, Jiangsu Province Hospital of Traditional Chinese MedicineAffiliated Hospital of Nanjing University of Chinese MedicineNanjingChina
  2. 2.The Second Clinical CollegeNanjing University of Chinese MedicineNanjingChina
  3. 3.Basic Medical SciencesNanjing University of Chinese MedicineNanjingChina

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