Molecular Neurobiology

, Volume 54, Issue 2, pp 922–929 | Cite as

miR-9 Mediates CALHM1-Activated ATP-P2X7R Signal in Painful Diabetic Neuropathy Rats

  • Wenjie Liu
  • Qinying Ao
  • Qulian Guo
  • Wanyou He
  • Liangyu Peng
  • Jun Jiang
  • Xiaoling Hu
Article
First Online:
Received:
Accepted:

Abstract

In this study, we planned to illuminate the mechanisms of the expression and function of CALHM1 in painful diabetic neuropathy (PDN). PDN rat model was constructed. The expression of CALHM1 and miR-9 in rat spinal dorsal horn neurons was detected. The correlation between the level of CALHM1 mRNA and 50 % PWT and the relationship between the expression of CALHM1 and miR-9 in rat spinal dorsal horn neurons were statistically analyzed. The effect of miR-9 and CALHM1 on each other’s expression in PDN rat spinal dorsal horn neurons were tested by qRT-PCR or Western blot. The co-culture system of neurons and glias from PDN rat spinal dorsal horn was constructed. The concentration of calcium and ATP as well as the expression of P2X7 receptor regulated by CALHM1 and miR-9 in PDN rat spinal dorsal horn neurons was measured. The results showed that the expression of CALHM1 was increased in PDN rat compared with controls, while its mRNA level was negatively correlated with 50 % PWT. miR-9, which was also upregulated in the spinal dorsal horn neurons of PDN rats, was positively correlated with the expression of CALHM1. The concentration of calcium and ATP as well as the expression of P2X7 receptor in glias was also increased in PDN rats. These increases could be reverted by inhibiting CALHM1 and/or miR-9. CALHM1 is involved in miR-9-mediated ATP-P2X7 pathway between neurons and glias in PDN rat.

Keywords

Diabetes mellitus Painful neuropathy CALHM1 miR-9 P2X7 receptor 
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Notes

Compliance with Ethical Standards

Animal procedures followed guidelines of Central South University Xiangya Hospital Animal Care Committee and Nanhua University Animal Care Committee.

Conflict of Interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Wenjie Liu
    • 1
  • Qinying Ao
    • 2
  • Qulian Guo
    • 3
  • Wanyou He
    • 4
  • Liangyu Peng
    • 1
  • Jun Jiang
    • 1
  • Xiaoling Hu
    • 1
  1. 1.Anesthesia DepartmentThe First Affiliated Hospital of Nanhua UniversityHengyangChina
  2. 2.Health SchoolThe Affiliated Nanhua Hospital of Nanhua UniversityHengyangChina
  3. 3.Anesthesia DepartmentXiangya Hospital of Central South UniversityChangshaChina
  4. 4.Anesthesia DepartmentThe First People’s Hospital of Foshan CityFoshanChina

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