Molecular Neurobiology

, Volume 54, Issue 10, pp 8050–8062 | Cite as

Enhancement of Neural Stem Cell Survival, Proliferation, Migration, and Differentiation in a Novel Self-Assembly Peptide Nanofibber Scaffold

  • Sajad Sahab Negah
  • Zabihollah Khaksar
  • Hadi Aligholi
  • Shahin Mohammad Sadeghi
  • Sayed Mostafa Modarres Mousavi
  • Hadi Kazemi
  • Ali Jahanbazi Jahan-Abad
  • Ali Gorji


Considerable efforts have been made to combine biologically active molecules into the self-assembling peptide in order to improve cells growth, survival, and differentiation. In this study, a novel three-dimensional scaffold (RADA4GGSIKVAV; R-GSIK) was designed by adding glycine and serine between RADA4 and IKVAV to promote the strength of the peptide. The cell adhesion, viability, proliferation, migration, and differentiation of rat embryonic neural stem cells (NSCs) in R-GSIK were investigated and compared to laminin-coated, two-dimensional, and Puramatrix cultures. The scanning electron microscopy studies of the R-GSIK showed an open porous structure and a suitable surface area available for cell interaction. R-GSIK promoted the cell adhesion, viability, proliferation, and migration compared to the other cultures. In addition, the R-GSIK enhanced NSCs differentiation into neuronal cells. The NSCs injected in R-GSIK had a lower glial differentiation rate than in the Puramatrix. The results suggest that R-GSIK holds great promise for cell therapies and neuronal tissue repair.


Stem cell therapy Tissue engineering Self-assembly Self-renewal ability 



This work was supported by the Shefa Neuroscience Center, Tehran, Iran, grant related to Dr-Thesis 31528 and Iran National Science Foundation, Tehran, Iran (INSF).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Sajad Sahab Negah
    • 1
    • 2
    • 3
  • Zabihollah Khaksar
    • 1
  • Hadi Aligholi
    • 4
  • Shahin Mohammad Sadeghi
    • 5
  • Sayed Mostafa Modarres Mousavi
    • 2
  • Hadi Kazemi
    • 6
  • Ali Jahanbazi Jahan-Abad
    • 2
    • 7
  • Ali Gorji
    • 2
    • 3
    • 8
    • 9
  1. 1.Histology and Embryology Group, Basic Science Department, Faculty of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Shefa Neuroscience Research CenterKhatam Alanbia HospitalTehranIran
  3. 3.Department of NeuroscienceMashhad University of Medical SciencesMashhadIran
  4. 4.Department of Neuroscience, School of Advanced Medical Sciences and TechnologiesShiraz University of Medical SciencesShirazIran
  5. 5.Department of Plastic and Reconstructive SurgeryShahid Beheshti University of Medical SciencesTehranIran
  6. 6.Pediatric Department, Medical FacultyShahed UniversityTehranIran
  7. 7.Department of Clinical BiochemistryShahid Beheshti University of Medical SciencesTehranIran
  8. 8.Department of Neurology and Department of NeurosurgeryWestfälische Wilhelms-Universität MünsterMünsterGermany
  9. 9.Epilepsy Research CenterWestfälische Wilhelms-Universität MünsterMünsterGermany

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