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Noggin Along with a Self-Assembling Peptide Nanofiber Containing Long Motif of Laminin Induces Tyrosine Hydroxylase Gene Expression

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An Erratum to this article was published on 30 August 2016

Abstract

Tyrosine hydroxylase (TH), a rate-limiting step in catecholamine synthesis in which its activity influences Alzheimer disease, Parkinson disease, and IQ of schizophrenia patients, has been studied for a long time. In the meantime, the present investigation assessed the effect of noggin and type of self-assembling nanofibers in TH gene over-expression by neuron-like cells derived from human endometrial-derived stromal cells (hEnSCs). Neuroblastoma cells and hEnSCs encapsulated into nanofibers including Matrigel, (RADA)4, laminin, and BMHP-1 motif bounded to (RADA)4 and their cell viability were studied for 48 h and 18 days in basal and neurogenic media, respectively, in noggin-rich media. Then, expression of neural genes and proteins has been investigated by immunocytochemistry (ICC) and real-time PCR methods, respectively. The results indicated that neuroblastoma cell and hEnSC viability is in good agreement with the level of Bcl2 and β-tubulin III gene expression; however, -BMHP-1 and -laminin nanofibers exhibited significantly higher cell viability eventually through Wnt/β-catenin signaling pathway as compared to others, respectively. The gene expression analysis of nanofibers showed that none of them induced gamma-aminobutyric acid (GABA) gene expression while glial fibrillary acidic protein (GFAP) gene just over-expressed in cells encapsulated into Matrigel with a low level of Bcl2 gene expression. However, the TH gene just had been over-expressed in cells encapsulated into -laminin nanofiber and 2D cell culture. In the absence of noggin with -laminin nanofibers, TH gene expression was suppressed. It might be concluded that although noggin through anti-BMP pathways resulted in GFAP decrement and TH gene increment, the type of scaffold that defined the final fate of cells and -laminin accompaniment might be useful for the recovery of Alzheimer and Parkinson disease patients.

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Acknowledgments

This work was supported by grant from the “Student’s Scientific Research Center, Tehran University of Medical Sciences, Iran (Grant number 92-02-61-22861).”

Author information

Authors and Affiliations

  1. Drug Nanocarriers Research Core, Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran

    Shima Tavakol

  2. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Shima Tavakol & Seyed Mahdi Rezayat

  3. Advance Sciences and Technologies Association, Student’s Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Shima Tavakol

  4. Shefa Neuroscience Research Center, Khatam al-Anbia Hospital, Tehran, Iran

    Sayed Mostafa Modaress Musavi

  5. School of Medicine, Kashan University of Medical Sciences, Isfahan, Iran

    Behnaz Tavakol

  6. Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Elham Hoveizi

  7. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Jafar Ai

  8. Brain and Spinal Injury Research Center, Imam Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Jafar Ai

  9. Department of Toxicology and Pharmacology, School of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran

    Seyed Mahdi Rezayat

  10. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Seyed Mahdi Rezayat

Authors
  1. Shima Tavakol
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  2. Sayed Mostafa Modaress Musavi
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  3. Behnaz Tavakol
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  4. Elham Hoveizi
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  5. Jafar Ai
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Correspondence to Shima Tavakol.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s12035-016-0069-y.

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Tavakol, S., Musavi, S.M.M., Tavakol, B. et al. Noggin Along with a Self-Assembling Peptide Nanofiber Containing Long Motif of Laminin Induces Tyrosine Hydroxylase Gene Expression. Mol Neurobiol 54, 4609–4616 (2017). https://doi.org/10.1007/s12035-016-0006-0

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  • DOI: https://doi.org/10.1007/s12035-016-0006-0

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