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Molecular Neurobiology

, Volume 54, Issue 1, pp 115–124 | Cite as

Autophagy Promotes Microglia Activation Through Beclin-1-Atg5 Pathway in Intracerebral Hemorrhage

  • Bangqing Yuan
  • Hanchao Shen
  • Li Lin
  • Tonggang Su
  • Lina Zhong
  • Zhao YangEmail author
Article

Abstract

Previous study demonstrates that intracerebral hemorrhage (ICH) promotes microglia activation and inflammation. However, the exact mechanism of microglia activation induced by ICH is not clear. In this experiment, microglia autophagy was examined using electron microscopy, conversion of light chain 3(LC3), and monodansylcadaverine (MDC) staining to detect autophagic vacuoles. We found that ICH induced microglia autophagy and activation. The suppression of autophagy using either pharmacologic inhibitors (3-methyladenine, bafilomycin A1) or RNA interference in essential autophagy genes (BECN1 and ATG5) decreased the microglia activation and inflammation in ICH. Moreover, autophagy inhibitors reduced brain damage in ICH. In conclusion, these data indicate that ICH contributes to microglia autophagic activation through BECN1 and ATG5 and provide the therapeutical strategy for ICH.

Keywords

Autophagy Microglia Beclin-1 Atg5 ICH 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC No. 81571179) and China Postdoctoral Science Foundation (No. 2015M580776).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Bangqing Yuan
    • 2
  • Hanchao Shen
    • 2
  • Li Lin
    • 2
  • Tonggang Su
    • 2
  • Lina Zhong
    • 1
  • Zhao Yang
    • 1
    Email author
  1. 1.Department of Neurology, Yongchuan HospitalChongqing Medical UniversityChongqingChina
  2. 2.Department of NeurosurgeryThe 476th Hospital of PLAFuzhouChina

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