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Molecular Neurobiology

, Volume 53, Issue 9, pp 5935–5947 | Cite as

Nitric Oxide Interacts with Caveolin-1 to Facilitate Autophagy-Lysosome-Mediated Claudin-5 Degradation in Oxygen-Glucose Deprivation-Treated Endothelial Cells

  • Jie Liu
  • John Weaver
  • Xinchun Jin
  • Yuan Zhang
  • Ji Xu
  • Ke J. Liu
  • Weiping LiEmail author
  • Wenlan LiuEmail author
Article

Abstract

Using in vitro oxygen-glucose deprivation (OGD) model, we have previously demonstrated that 2-h OGD induces rapid, caveolin-1-mediated dissociation of claudin-5 from the cellular cytoskeletal framework and quick endothelial barrier disruption. In this study, we further investigated the fate of translocated claudin-5 and the mechanisms by which OGD promotes caveolin-1 translocation. Exposure of bEND3 cells to 4-h OGD, but not 2-h OGD plus 2-h reoxygenation, resulted in claudin-5 degradation. Inhibition of autophagy or the fusion of autophagosome with lysosome, but not proteasome, blocked OGD-induced claudin-5 degradation. Moreover, knockdown of caveolin-1 with siRNA blocked OGD-induced claudin-5 degradation. Western blot analysis showed a transient colocalization of caveolin-1, claudin-5, and LC3B in autolysosome or lipid raft fractions at 2-h OGD. Of note, inhibiting autophagosome and lysosome fusion sustained the colocalization of caveolin-1, claudin-5, and LC3B throughout the 4-h OGD exposure. EPR spin trapping showed increased nitric oxide (NO) generation in 2-h OGD-treated cells, and inhibiting NO with its scavenger C-PTIO or inducible nitric oxide synthase (iNOS) inhibitor 1400W prevented OGD-induced caveolin-1 translocation and claudin-5 degradation. Taken together, our data provide a novel mechanism underlying endothelial barrier disruption under prolonged ischemic conditions, in which NO promotes caveolin-1-mediated delivery of claudin-5 to the autophagosome for autophagy-lysosome-dependent degradation.

Keywords

Oxygen-glucose deprivation Claudin-5 Caveolin-1 Autophagy Lysosome 

Notes

Acknowledgments

We thank the financial support from the National Natural Science Foundation of China, the National Institutes of Health, and Shenzhen Science and Technology Innovation Commission. Details of funding support are listed below.

Compliance with Ethical Standards

Funding

This work was supported by grants from the National Natural Science Foundation of China (81371328), Shenzhen Science and Technology Innovation Commission (CXZZ20130516152706040 and ZDSY20140509173142601), and the National Institutes of Health (P20RR15636).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Translational Center for Stem Cell Research, Tongji Hospital, Stem Cell Research CenterTongji University School of MedicineShanghaiChina
  2. 2.The Central LaboratoryShenzhen Second People’s Hospital, Shenzhen University 1st Affiliated HospitalShenzhenChina
  3. 3.Department of Neurosurgery and Shenzhen Key Laboratory of NeurosurgeryShenzhen Second People’s Hospital, Shenzhen University 1st Affiliated HospitalShenzhenChina
  4. 4.Department of Pharmaceutical Sciences, College of PharmacyUniversity of New MexicoAlbuquerqueUSA
  5. 5.Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and Institute of Neuroscience, the Second Affiliated Hospital of Soochow UniversitySoochow UniversitySuzhouChina

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