Differentiation Potential of Human Chorion-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells in Two- and Three-Dimensional Culture Systems
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Many people worldwide suffer from motor neuron-related disorders such as amyotrophic lateral sclerosis and spinal cord injuries. Recently, several attempts have been made to recruit stem cells to modulate disease progression in ALS and also regenerate spinal cord injuries. Chorion-derived mesenchymal stem cells (C-MSCs), used to be discarded as postpartum medically waste product, currently represent a class of cells with self renewal property and immunomodulatory capacity. These cells are able to differentiate into mesodermal and nonmesodermal lineages such as neural cells. On the other hand, gelatin, as a simply denatured collagen, is a suitable substrate for cell adhesion and differentiation. It has been shown that electrospinning of scaffolds into fibrous structure better resembles the physiological microenvironment in comparison with two-dimensional (2D) culture system. Since there is no report on potential of human chorion-derived MSCs to differentiate into motor neuron cells in two- and three-dimensional (3D) culture systems, we set out to determine the effect of retinoic acid (RA) and sonic hedgehog (Shh) on differentiation of human C-MSCs into motor neuron-like cells cultured on tissue culture plates (2D) and electrospun nanofibrous gelatin scaffold (3D).
KeywordsMotor neuron Chorion Mesenchymal stem cells Electrospinning Gelatin
This study was financially supported by grant no. 92004328 from Iran National Science Foundation (INSF) and Cellular-Molecular Research Center of Iran University of Medical Sciences.
Conflict of Interest
The authors declare that they have no conflict of interest.
Compliance with Ethical Standards
After full-term delivery, human chorion-derived mesenchymal stem cells were collected from placenta tissues of new born babies. The protocol to obtain and use of the cells in research were approved by the ethical committee of Iran University of Medical Sciences, after obtaining maternal informed consent.
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