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Molecular Neurobiology

, Volume 53, Issue 2, pp 1132–1144 | Cite as

Effects of hnRNP A2/B1 Knockdown on Inhibition of Glioblastoma Cell Invasion, Growth and Survival

  • Jinmu Deng
  • Song Chen
  • Feng Wang
  • Hongxin Zhao
  • Zongyi Xie
  • Zhongye Xu
  • Qingtao Zhang
  • Ping Liang
  • Xuan Zhai
  • Yuan ChengEmail author
Article

Abstract

Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) plays an important role in influence of pre-messenger RNA (pre-mRNA) processing and mRNA metabolism and transportation in cells. Increasing evidence indicates that hnRNP A2/B1 played an important role in development and progression of various human cancers. Forty cases of normal and human glioma tissue samples were analyzed using immunohistochemistry to reveal the expression of hnRNP A2/B1 protein in the samples. Then, knockdown of hnRNP A2/B1 expression induced by RNA interference (RNAi) method was used to analyze the role of hnRNP A2/B1 in glioblastoma cell viability, adhesion, migration, invasion, and chemoresistance for temozolomide (TMZ). The data showed that hnRNP A2/B1 protein was overexpressed in glioma tissue specimens and associated with advanced glioma grades. Knockdown of hnRNP A2/B1 could reduce glioblastoma cell viability, adhesion, migration, invasion, and chemoresistance for TMZ capacity, but induced tumor cells to apoptosis and reactive oxygen species (ROS) generation in glioma U251 and SHG44 cells. Molecularly, hnRNP A2/B1 knockdown reduced expression of phospho-STAT3 and MMP-2. Detection of hnRNP A2/B1 expression may be useful as a biomarker for prediction of glioma progression and knockdown of hnRNP A2/B1 expression as a novel strategy in future control of glioblastoma in clinic.

Keywords

Glioblastoma hnRNP A2/B1 Cell growth Cell invasion shRNA 

Notes

Grant Support

This work is supported by the National Natural Science Foundation of China (No. 30870723 and 81201066) and Chongqing Natural Science Foundation Project (No. CSTC2013jcyjA10054).

Conflict of Interest

No potential conflicts of interest were disclosed.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Jinmu Deng
    • 1
  • Song Chen
    • 2
  • Feng Wang
    • 3
  • Hongxin Zhao
    • 4
  • Zongyi Xie
    • 3
  • Zhongye Xu
    • 3
  • Qingtao Zhang
    • 5
  • Ping Liang
    • 1
  • Xuan Zhai
    • 1
  • Yuan Cheng
    • 3
    Email author
  1. 1.Department of NeurosurgeryChildren’s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Ministry of Education Key Laboratory of Child Development and DisordersChongqingPeople’s Republic of China
  2. 2.Department of Neurosurgery, First Affiliated HospitalChongqing Medical UniversityChongqingPeople’s Republic of China
  3. 3.Department of Neurosurgery, Second Affiliated HospitalChongqing Medical UniversityChongqingPeople’s Republic of China
  4. 4.Department of Neurosurgery, First Affiliated HospitalZunyi Medical CollegeZunyiPeople’s Republic of China
  5. 5.Department of NeurosurgeryChongqing Emergency Medical CenterChongqingPeople’s Republic of China

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