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Molecular Neurobiology

, Volume 53, Issue 1, pp 369–378 | Cite as

Strategy to Suppress Oxidative Damage-Induced Neurotoxicity in PC12 Cells by Curcumin: the Role of ROS-Mediated DNA Damage and the MAPK and AKT Pathways

  • Xiao-yan Fu
  • Ming-feng Yang
  • Ming-zhi Cao
  • Da-wei Li
  • Xiao-yi Yang
  • Jing-yi Sun
  • Zong-yong Zhang
  • Lei-lei Mao
  • Shuai Zhang
  • Feng-ze Wang
  • Feng Zhang
  • Cun-dong FanEmail author
  • Bao-liang SunEmail author
Article

Abstract

Oxidative damage plays a key role in causation and progression of neurodegenerative diseases. Inhibition of oxidative stress represents one of the most effective ways in treating human neurologic diseases. Herein, we evaluated the protective effect of curcumin on PC12 cells against H2O2-induced neurotoxicity and investigated its underlying mechanism. The results indicated that curcumin pre-treatment significantly suppressed H2O2-induced cytotoxicity, inhibited the loss of mitochondrial membrane potential (Δψm) through regulation of Bcl-2 family expression, and ultimately reversed H2O2-induced apoptotic cell death in PC12 cells. Attenuation of caspase activation, poly(ADP-ribose) polymerase (PARP) cleavage, DNA damage, and accumulation of reactive oxygen species (ROS) all confirmed its protective effects. Moreover, curcumin markedly alleviated the dysregulation of the MAPK and AKT pathways induced by H2O2. Taken together, our findings suggest that the strategy of using curcumin could be a highly effective way in combating oxidative damage-mediated human neurodegenerative diseases.

Keywords

Curcumin Oxidative damage Apoptosis Reactive oxygen species Neurodegenerative diseases 

Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81471212, 81271275, 81070947, and 30770759 to B.-L. Sun; No. 81271276 to F. Zhang) and by the Natural Science Foundation of Shandong (No. ZR2012HZ006 to B.-L. Sun).

Supplementary material

12035_2014_9021_MOESM1_ESM.doc (53 kb)
ESM 1 (DOC 53 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Xiao-yan Fu
    • 1
    • 2
  • Ming-feng Yang
    • 2
  • Ming-zhi Cao
    • 3
  • Da-wei Li
    • 2
  • Xiao-yi Yang
    • 2
  • Jing-yi Sun
    • 1
  • Zong-yong Zhang
    • 2
  • Lei-lei Mao
    • 2
  • Shuai Zhang
    • 2
  • Feng-ze Wang
    • 2
  • Feng Zhang
    • 2
    • 4
  • Cun-dong Fan
    • 2
    Email author
  • Bao-liang Sun
    • 2
    • 5
    Email author
  1. 1.School of Basic MedicineTaishan Medical UniversityTaianChina
  2. 2.Key Lab of Cerebral Microcirculation in Universities of ShandongTaishan Medical UniversityTaianChina
  3. 3.Department of Neurosurgery, Huxi HospitalJining Medical UniversityShanxianChina
  4. 4.Department of Neurology and Center of Cerebrovascular Disease ResearchUniversity of PittsburghPittsburghUSA
  5. 5.Department of NeurologyAffiliated Hospital of Taishan Medical UniversityTaianChina

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