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Molecular Neurobiology

, Volume 52, Issue 3, pp 1704–1713 | Cite as

Evaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds

  • Somayeh Ebrahimi-Barough
  • Abbas Norouzi Javidan
  • Hoshangh Saberi
  • Mohammad Tghi Joghataei
  • Reza Rahbarghazi
  • Esmaeil Mirzaei
  • Faezeh Faghihi
  • Sadegh Shirian
  • Armin Ai
  • Jafar Ai
Article

Abstract

Human endometrium is a high-dynamic tissue that contains human endometrial stem cells (hEnSCs) which can be differentiated into a number of cell lineages. The differentiation of hEnSCs into many cell lineages such as osteoblast, adipocyte, and neural cells has been investigated previously. However, the differentiation of these stem cells into motor neuron-like cells has not been investigated yet. Different biochemical and topographical cues can affect the differentiation of stem cells into a specific cell. The aim of this study was to investigate the capability of hEnSCs to be differentiated into motor neuron-like cells under biochemical and topographical cues. The biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) electrospun nanofibrous scaffold was used as a topographical cue. Human EnSCs were cultured on the PLGA scaffold and tissue culture polystyrene (TCP), then differentiation of hEnSCs into motor neuron-like cells under induction media including retinoic acid (RA) and sonic hedgehog (Shh) were evaluated for 15 days. The proliferation rate of cells was assayed by using MTT assay. The morphology of cells was studied by scanning electron microscopy imaging, and the expression of motor neuron-specific markers by real-time PCR and immunocytochemistry. Results showed that survival and differentiation of hEnSCs into motor neuron-like cells on the PLGA scaffold were better than those on the TCP group. Taken together, the results suggest that differentiated hEnSCs on PLGA can provide a suitable, three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system, and these cells may be a potential candidate in cellular therapy for motor neuron diseases.

Keywords

Human endometrial stem cell Motor neuron differentiation Nanofibrous scaffolds PLGA 

Notes

Acknowledgments

We thank the Iran National Science Foundation (INSF) for the financial support (grant number 92004774) and Tehran University of Medical Sciences for this research.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Somayeh Ebrahimi-Barough
    • 1
    • 2
  • Abbas Norouzi Javidan
    • 1
  • Hoshangh Saberi
    • 1
  • Mohammad Tghi Joghataei
    • 3
    • 4
  • Reza Rahbarghazi
    • 5
  • Esmaeil Mirzaei
    • 6
  • Faezeh Faghihi
    • 3
  • Sadegh Shirian
    • 1
  • Armin Ai
    • 7
  • Jafar Ai
    • 1
    • 2
  1. 1.Brain and Spinal Cord Injury Research Center (BASIR)Tehran University of Medical SciencesTehranIran
  2. 2.Department of Tissue Engineering, Faculty of Advanced Technologies in MedicineTehran University of Medical SciencesTehranIran
  3. 3.Cellular and Molecular Research CenterIran University of Medical SciencesTehranIran
  4. 4.Department of Anatomy, School of MedicineIran University of Medical SciencesTehranIran
  5. 5.Umbilical Cord Stem Cell Research CenterTabriz University of Medical SciencesTabrizIran
  6. 6.Department of Medical Nanotechnology, School of Advanced Technologies in MedicineTehran University of Medical SciencesTehranIran
  7. 7.Dentistry FacultyTehran University of Medical SciencesTehranIran

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